NDLI: Fluorescence Spectroscopic Analysis of the Interaction of Papain with Ionic Liquids

Content Provider	Springer Nature Link
Author	Fan, Yunchang Yan, Jinlong Zhang, Sheli Li, Junhai Chen, Dong Duan, Peigao
Copyright Year	2012
Abstract	The interaction between papain and two typical ionic liquids (ILs), 1-octyl-3-methylimidazolium chloride ([C$_{8}$mim]Cl) and 1-butyl-3-methylimidazolium chloride ([C$_{4}$mim]Cl), was investigated by using fluorescence spectroscopy technique at a pH value of 7.4. The results suggested that ILs could quench the intrinsic fluorescence of papain probably via a static quenching mechanism. The binding constants were determined by employing the fluorescence quenching method. They were very small compared with that of volatile organic solvents, indicating that only very weak interaction between ILs and papain existed. The Gibbs free energy change (∆G), enthalpy change (∆H), and entropy change (∆S) during the interaction of papain and ILs were estimated. Negative values of these parameters indicated that the interaction between ILs and papain was a spontaneous process, also implying that hydrogen bonding and van der Waals forces played important roles in the interaction processes. Figure Three-dimensional fluorescence spectrum of papain (0.2 g L-1)
Starting Page	592
Ending Page	603
Page Count	12
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	168
Issue Number	3
e-ISSN	15590291
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-07-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ionic liquids (ILs) Fluorescence spectroscopy Binding mechanism Papain Thermodynamic parameters Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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