NDLI: Differential Regulation of Defense-Related Gene Expression in Response to Red Rot Pathogen Colletotrichum falcatum Infection in Sugarcane

Content Provider	Springer Nature Link
Author	Prathima, P. T. Raveendran, M. Kumar, K. K. Rahul, P. R. Kumar, V. Ganesh Viswanathan, R. Sundar, A. Ramesh Malathi, P. Sudhakar, D. Balasubramaniam, P.
Copyright Year	2013
Abstract	Red rot is a serious disease of sugarcane caused by the fungus Colletotrichum falcatum imposing a considerable economic loss annually in all sugarcane-producing countries. In this study, we analyzed the early resistance response of sugarcane to red rot fungus by comparing the differences between control and inoculated stalk tissues. Differential display reverse transcription polymerase chain reaction (DD-RT-PCR) was employed to identify altered expression of genes in disease-resistant cv Co 93009, in response to pathogen infection. DD-RT-PCR identified 300 differentially expressed transcripts of which 112 were selected for further analysis. Cloning and sequence analysis of the isolated cDNA fragments resulted in functional categorization of these clones into five categories, of which the defense/stress/signaling group was the largest, with clones homologous to genes known to be actively involved in various pathogenesis-related functions in plant species. This group showed overexpression of several transcripts related to ethylene-mediated and jasmonic acid pathway of plant defense mechanisms. Of the 112 expressed sequence tags, validation of expression was carried out for five important genes whose role in plant defense mechanisms is well established. This is the first report of Colletotrichum-mediated gene regulation in sugarcane which has provided a set of candidate genes for detailed molecular dissection of signaling and defense responses in tropical sugarcane during the onset of red rot resistance.
Starting Page	488
Ending Page	503
Page Count	16
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	171
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2013-07-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Differential display C. falcatum–sugarcane interaction Red rot Host–pathogen interaction Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Differential regulation of defense-related gene expression in response to red rot pathogen Colletotrichum falcatum infection in sugarcane.

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