NDLI: Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Content Provider	Springer Nature Link
Author	Zhao, Junqi Guo, Chao Tian, Chaoguang Ma, Yanhe
Copyright Year	2015
Abstract	A novel β-glucosidase of glycoside hydrolase (GH) family 3 from Myceliophthora thermophila (mtbgl3b) was successfully expressed in Pichia pastoris. The full-length gene consists of 2613 bp nucleotides encoding a protein of 870 amino acids. MtBgl3b showed maximum activity at pH 5.0 and remained more than 70 % relative activity at 3.5–6.0. The enzyme displayed the highest activity at 60 °C and kept about 90 % relative activity for 50–65 °C; besides, the enzyme showed psychrophilic trait and remains 51 % relative activity at 40 °C. MtBgl3b exhibited good stability over a wide pH range of 3.0–10.0 and was thermostable at 60 and 65 °C. The enzyme displayed highest activity towards p-nitrophenyl-β-d-glucopyranoside (pNPG), followed by p-nitrophenyl-d-cellobioside (pNPC), cellotetraose, cellotriose, cellobiose, and gentiobiose. When using 10 % cellobiose (w/v) as the substrate, the enzyme showed transglycosylation activity to produce the cellotriose. The kinetic parametric of K $_{m}$ and V $_{max}$ were 2.78 mM and 927.9 μM mg$^{−1}$ min$^{−1}$, respectively. Finally, the reaction mode of the enzyme and the substrates were analyzed by molecular docking approach.
Starting Page	511
Ending Page	527
Page Count	17
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	177
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2015-08-04
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Myceliophthora thermophila β-Glucosidase Substrate specificity Transglycosylation activity Pichia pastoris Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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