NDLI: Isolation, Purification, and Characterization of Two Thermostable Endo-1,4-β-d-glucanase Forms from Opuntia vulgaris

Content Provider	Springer Nature Link
Author	Shyamala, Sivalingam Ravikumar, Sambandam Vikramathithan, Jeyaraman Srikumar, Kotteazeth
Copyright Year	2011
Abstract	Four endoglucanase temperature isoforms (T $_{30}$, T $_{50}$, T $_{70}$, and T $_{90}$) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, respectively, on gel filtration chromatography. These isoforms were purified 24-, 25-, 29-, and 27-fold with a yield of 15%, 12%, 17%, and 19% and having a specific activity of 120, 125, 144, and 136 U/mg, respectively. The thermostable T $_{70}$ and T $_{90}$ isoforms exhibited optimum activity at pH 4.5 and 7 and also yielded a molecular weight of 66 and 36 kDa, respectively, as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The T $_{70}$ had a K $_{m}$ of 43 mM and a V $_{max}$ of 12.5 μmol min$^{−1}$ μg$^{−1}$ of protein, and the T $_{90}$ isoform had a K $_{m}$ of 40 mM, with an apparent V $_{max}$ of 10 μmol min$^{−1}$ μg$^{−1}$ of protein. Western blot, immunodiffusion, and in vitro inhibition assays established the reactivity of the T $_{90}$ isoform with polyclonal anti-T $_{90}$ antibody raised in rabbit. Cross-reactivity of this antibody with the T $_{70}$ endoglucanase isoform was also noted.
Starting Page	1597
Ending Page	1610
Page Count	14
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	165
Issue Number	7-8
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-09-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulases Endoglucanase Thermophilic Metal ion Opuntia vulgaris Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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