NDLI: Citrate Stimulates Oligosaccharide Synthesis in Metabolically Engineered Agrobacterium sp.

Content Provider	Springer Nature Link
Author	Ruffing, Anne M. Chen, Rachel Ruizhen
Copyright Year	2011
Abstract	Agrobacterium sp. ATCC 31749 was previously shown to be an advantageous host for oligosaccharide production. Unexpectedly, the addition of citrate to the oligosaccharide synthesis reaction resulted in up to a sixfold improvement in the production N-aceytl-lactosamine, a disaccharide. The possible mechanisms for this citrate-induced stimulation of oligosaccharide production were investigated, including the consumption of citrate as a carbon and energy source, enhanced metal ion solubility from citrate chelation, and the ability of citrate to act as a buffer. The main mechanisms for the effect of citrate on oligosaccharide production were determined to be carbon and energy provision from citrate consumption and pH maintenance. ATCC 31749 was shown to co-metabolize citrate along with sucrose, a preferred carbon source, indicating the lack of a catabolite repression system in this Agrobacterium. Metabolic flux analysis suggested an increase in flux through TCA cycle for the citrate-containing reaction, which may provide additional energy supply to support enhanced oligosaccharide production. The citrate stimulation of oligosaccharide synthesis was shown to be unique to the Agrobacterium strain, as a similarly engineered Escherichia coli strain did not show significant improvement in oligosaccharide production with citrate addition. This work provides insight into the metabolism of Agrobacterium sp. ATCC 31749 and highlights important factors in whole-cell oligosaccharide synthesis.
Starting Page	851
Ending Page	866
Page Count	16
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	164
Issue Number	6
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-02-08
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oligosaccharide synthesis Agrobacterium ATCC 31749 Whole-cell synthesis Metabolic flux analysis Citrate Co-metabolism of citrate Catabolite repression Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Citrate stimulates oligosaccharide synthesis in metabolically engineered Agrobacterium sp.

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Citrate Stimulates Oligosaccharide Synthesis in Metabolically Engineered Agrobacterium sp.

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Citrate Stimulates Oligosaccharide Synthesis in Metabolically Engineered Agrobacterium sp.