NDLI: Improvement of Aspergillus sulphureus Endo-β-1,4-Xylanase Expression in Pichia pastoris by Codon Optimization and Analysis of the Enzymic Characterization

Content Provider	Springer Nature Link
Author	Li, Yihang Zhang, Bo Chen, Xiang Chen, Yiqun Cao, Yunhe
Copyright Year	2009
Abstract	The gene xynB from Aspergillus sulphureus encoding the endo-β-1,4-xylanase was de novo synthesized by splicing overlap extension polymerase chain reaction according to Pichia pastoris protein’s codon bias. The synthetic DNA and wild-type DNA were placed under the control of a glyceraldehyde-3-phosphate dehydrogenase gene promoter (GAP) in the constitutive expression vector plasmid pGAPzαA and electrotransformed into the P. pastoris X-33 strain, respectively. The transformants screened by Zeocin were able to constitutively secrete the xylanase in YPD liquid medium. The maximum yield of the recombinant xylanase produced by the synthetic DNA was 105 U ml$^{−1}$, which was about 5-fold higher than that by wild-type DNA under the flask culture at 28 °C for 3 days. The enzyme showed optimal activity at 50 °C and pH 5.0. The residual activity remained above 90% after the recombinant xylanase was pretreated in Na$_{2}$HPO$_{4}$–citric acid buffer (pH 2.4) for 2 h. The xylanase activity was significantly improved by Zn$^{2+}$. These biochemical characteristics suggest that the recombinant xylanase has a prospective application in feed industry as an additive.
Starting Page	1321
Ending Page	1331
Page Count	11
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	160
Issue Number	5
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2009-05-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endo-β-1,4-xylanase Aspergillus sulphureus De novo synthesis Codon optimization Pichia pastoris Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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