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Optimization of Supercritical Carbon Dioxide Extraction of Bioactive Flavonoid Compounds from Spearmint (Mentha spicata L.) Leaves by Using Response Surface Methodology

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Optimization of Supercritical Carbon Dioxide Extraction of Bioactive Flavonoid Compounds from Spearmint (Mentha spicata L.) Leaves by Using Response Surface Methodology

Content Provider	Springer Nature Link
Author	Bimakr, Mandana Rahman, Russly Abdul Ganjloo, Ali Taip, Farah Saleena Salleh, Liza Md Sarker, Md Zaidul Islam
Copyright Year	2011
Abstract	The bioactive flavonoid compounds of spearmint (Mentha spicata L.) leaves were obtained by using supercritical carbon dioxide (SC-CO$_{2}$) extraction. Extraction was carried out according to face-centred central composite design, and independent variables were pressure (100, 200 and 300 bar), temperature (40, 50 and 60 °C) and co-solvent amount (3, 6 and 9 g/min). The extraction process was optimized by using response surface methodology for the highest crude extraction yield of bioactive flavonoid compounds. The optimal conditions were identified as 209.39 bar pressure, 50.00 °C temperature and 7.39 g/min co-solvent amount. The obtained extract under optimum SC-CO$_{2}$ condition was analysed by high-performance liquid chromatography. Seven bioactive flavonoids including catechin, epicatechin, rutin, luteolin, myricetin, apigenin and naringenin were identified as major compounds. The results of quantification showed that spearmint leaves are potential source of antioxidant compounds.
Starting Page	912
Ending Page	920
Page Count	9
File Format	PDF
ISSN	19355130
Journal	Food and Bioprocess Technology
Volume Number	5
Issue Number	3
e-ISSN	19355149
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-01-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Spearmint (Mentha spicata L.) Supercritical carbon dioxide (SC-CO$_{2}$) extraction Response surface methodology Bioactive flavonoid compounds HPLC Biotechnology Food Science Chemistry/Food Science Agriculture
Content Type	Text
Resource Type	Article
Subject	Industrial and Manufacturing Engineering Safety, Risk, Reliability and Quality Food Science Process Chemistry and Technology

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Optimization of Supercritical Carbon Dioxide Extraction of Bioactive Flavonoid Compounds from Spearmint (Mentha spicata L.) Leaves by Using Response Surface Methodology

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