NDLI: Aqueous Two-Phase Micellar System for Nisin Extraction in the Presence of Electrolytes

Content Provider	Springer Nature Link
Author	Jozala, Angela Fausti Lopes, André Moreni Lencastre vaes, Letícia Celia Mazzola, Priscila Gava Penna, Thereza Christina Vessoni Júnior, Adalberto Pessoa
Copyright Year	2012
Abstract	Liquid–liquid extraction for aqueous two-phase micellar systems (ATPMS) is a promising technique that can either replace or be used as a complementary process to more typical chromatographic operations in order to reduce the costs of downstream processing of many biological products. This method offers attractive conditions when exploited in the extraction/purification of a target protein directly on the culture medium. Nisin, an extracellular antimicrobial peptide, is produced by Lactococcus lactis and is effective at controlling a wide range of Gram-positive bacteria, including multidrug-resistant pathogens. This study evaluates ATPMS composed by a nonionic surfactant, Triton X-114, in the presence or absence of electrolytes, to improve the extraction of nisin. The partitioning behavior of nisin showed that it can be directly extracted from the fermentation media. The partitioning coefficient (K $_{nis}$) of the commercial nisin in the presence of electrolytes showed K $_{nis}$ values of 5.6 and 5.4 for MgSO$_{4}$ and (NH$_{4}$)$_{2}$SO$_{4}$, respectively. Similar behavior was observed for the produced nisin where K $_{nis}$ values were 4.1 and 5.1 for MgSO$_{4}$ and (NH$_{4}$)$_{2}$SO$_{4}$. After partition, the commercial nisin activity in the micelle-rich phase, in the absence of electrolytes, was 3.3 logAU/mL, and in the presence of MgSO$_{4}$ and (NH$_{4}$)$_{2}$SO$_{4}$, the activity was 5.0 logAU/mL. The produced nisin activity with and without MgSO$_{4}$ was around 3.5 logAU/mL; however, in the presence of (NH$_{4}$)$_{2}$SO$_{4}$, nisin activity was 4.5 logAU/mL. The increase in nisin activity after partitioning with salts encourages further researches for the optimization of nisin extraction.
Starting Page	3456
Ending Page	3461
Page Count	6
File Format	PDF
ISSN	19355130
Journal	Food and Bioprocess Technology
Volume Number	6
Issue Number	12
e-ISSN	19355149
Language	English
Publisher	Springer US
Publisher Date	2012-12-04
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Liquid–liquid extraction Electrolytes Lactococcus lactis Peptide purification Nisin Triton X-114 Food Science Chemistry/Food Science Agriculture Biotechnology
Content Type	Text
Resource Type	Article
Subject	Industrial and Manufacturing Engineering Safety, Risk, Reliability and Quality Food Science Process Chemistry and Technology

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