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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 11

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Identification of human liver cytochromes P450 by using MALDI-TOF mass spectrometry

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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 1

Identification of human liver cytochromes P450 by using MALDI-TOF mass spectrometry

Content Provider	Springer Nature Link
Author	Petushkova, N. A. Lisitsa, A. V. Karuzina, I. I. Zgoda, V. G. Sheremetyeva, G. F. Samenkova, N. F. Nikitin, I. P. Sakharova, T. A. Kopylov, A. T. Archakov, A. I.
Copyright Year	2008
Abstract	Proteomic approaches have been used for detection and identification of cytochromes P450 forms from highly purified membrane preparations of human liver. These included the protein separation by 2D-and/or 1D-electrophoresis and molecular scanning of a SDS-PAGE gel fragment in a range 45–66 kDa (this area corresponds molecular weights of cytochromes P450). The analysis of protein content was statistically evaluated by means of an original 1D-ZOOMER software package which allowed to carry out the processing of mass spectra mixture instead of individual mass spectra used by standard techniques. In the range 45–66 kDa we identified 13 microsomal membrane proteins including such cytochrome P450 forms as CYPs 1A2, 1B1, 2A6, 2E1, 2C8, 2C9, 2C10, 2D6, 3A4, 4A11, 4F2. Study of enzymatic activities of human liver microsomal cytochrome P450 isoforms CYP 1A, 2B, 3A, and 2E revealed the decrease in the rates of O-dealkylation and N-demethylation catalyzed by CYP 450 1A1/1A2 and 3A4 under pathological conditions, whereas 7-benzyloxyresorufin-O-debenzylase activity (which characterizes the total activity of CYP 2B and CYP 2C), the activities of CYP 2E1 (methanol oxidation), 7-pentoxyresorufin-O-dealkylation (CYP 2B), 7-ethoxy-and 7-methoxycoumarin-O-dealkylases (CYP 2B1) remained basically unchanged.
Starting Page	47
Ending Page	54
Page Count	8
File Format	PDF
ISSN	19907508
Journal	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Volume Number	2
Issue Number	1
e-ISSN	19907516
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2008-04-03
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	microsomes liver cytochromes P450 microsomal oxidation one dimensional (1D) and two dimensional (2D) electrophoresis mass spectrometry Bioorganic Chemistry Medicinal Chemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Clinical Biochemistry Molecular Medicine

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