NDLI: The interaction of human cytokeratins with isatin analogues

Content Provider	Springer Nature Link
Author	Buneeva, O. A. Gnedenko, O. V. Fedchenko, V. I. Ivav, A. S. Medvedev, A. E.
Copyright Year	2010
Abstract	Using an optical biosensor Biacore 3000 the interaction of human recombinant cytokeratins (CK) with isatin analogues (5-aminocaproyl-isatin and 5-aminoisatin) immobilized on the CM5 chip has been investigated. CK-14 effectively interacted with 5-aminocaproyl-isatin immobilized on the carboxymethyl dextran chip surface, but not with a “shorter” analogue (5-aminoisatin). In contrast to CK14, CK8 effectively interacted only with 5-aminoisatin. In both cases cytokeratin binding with the immobilized isatin analogues was characterized by rather high affinity (K$_{d}$ of 0.7 μM for the pair CK14/immobilized 5-aminocaproylisatin and 1.7 μM for the pair CK8/immobilized 5-aminoisatin). CK20 did not interact with both immobilized isatin analogues. Taking into consideration non-specific binding of mouse CK14 and rat CK8 with 5-aminocaproyl-isatin Sepharose we have performed comparative analysis of amino acid sequences of human, mouse, and rat CK8 and CK14. The data obtained suggest that in the case of human, mouse, and rat CK14 the N-terminal domain is the most variable among these species, whereas the major differences between amino acid sequences of human, mouse, and rat CK8 have been found both in N-terminal and C-terminal regions.
Starting Page	107
Ending Page	111
Page Count	5
File Format	PDF
ISSN	19907508
Journal	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Volume Number	4
Issue Number	1
e-ISSN	19907516
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-03-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	isatin isatin analogues isatin binding proteins cytokeratins optical biosensor protein-ligand interactions Medicinal Chemistry Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Clinical Biochemistry Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Oxidative modification of glyceraldehyde-3-phosphate dehydrogenase influences its interaction with endogenous neuroprotector isatin

Optical surface plasmon resonance biosensors in molecular fishing

Interaction of pyruvate kinase with isatin and deprenyl

The optical biosensor study of the redox partner interaction with the cytochrome P450 2B4-containing monooxygenase system under hydroxylation conditions

Protocols of protein interactomics: Molecular fishing on optical chips and magnetic nanoparticles

The use of immobilized ubiquitin for biosensor analysis of the mitochondrial subinteractome

Ligand–Protein Interaction in Plant Seed Thiamine-Binding Proteins. Binding of Various Thiamine Analogues to the Sepharose-Immobilized Buckwheat-Seed Protein

Biosensor analysis of the interaction of potential dimerization inhibitors with HIV-1 protease

Inhibition of NO-dependent soluble human platelet guanylate cyclase by isatin

The interaction of human cytokeratins with isatin analogues

Similar Documents

Oxidative modification of glyceraldehyde-3-phosphate dehydrogenase influences its interaction with endogenous neuroprotector isatin

Optical surface plasmon resonance biosensors in molecular fishing

Interaction of pyruvate kinase with isatin and deprenyl

The optical biosensor study of the redox partner interaction with the cytochrome P450 2B4-containing monooxygenase system under hydroxylation conditions

Protocols of protein interactomics: Molecular fishing on optical chips and magnetic nanoparticles

The use of immobilized ubiquitin for biosensor analysis of the mitochondrial subinteractome

Ligand–Protein Interaction in Plant Seed Thiamine-Binding Proteins. Binding of Various Thiamine Analogues to the Sepharose-Immobilized Buckwheat-Seed Protein

Biosensor analysis of the interaction of potential dimerization inhibitors with HIV-1 protease

Inhibition of NO-dependent soluble human platelet guanylate cyclase by isatin

The interaction of human cytokeratins with isatin analogues