NDLI: Atomic force microscopy of supported lipid membranes and their complexes with polycations

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Atomic force microscopy of supported lipid membranes and their complexes with polycations

Content Provider	Springer Nature Link
Author	Sybachin, A. V. Tsarkova, L. A. Yaroslavov, A. A.
Copyright Year	2010
Abstract	The method of atomic force microscopy has been used to investigate the morphology of mica-supported bilayer lipid membranes and stability of their complexes with a cationic polymer, poly-(N-ethyl-4-vinylpyridinium bromide). Lipid bilayers with a minimum of defects were obtained by the fusion of monolamellar neutral or mixed anionic bilayer vesicles (liposomes) on the mica surface, followed by excessive solvent removal by means of rapid rotation of a plate in horizontal plane (spin-coating). It has been shown that the cationic polymer does not interact with the bilayers, where the outer leaflet (i.e., the monolayer exposed to the surrounding aqueous solution) is made of an electroneutral phosphatidylcholine (PC). At the same time, the polymer irreversibly binds to the bilayer containing an anionic lipid.
Starting Page	240
Ending Page	246
Page Count	7
File Format	PDF
ISSN	19907478
Journal	Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Volume Number	4
Issue Number	2
e-ISSN	19907494
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-07-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	lipid bilayers cationic polymer liposomes Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Biophysics

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