NDLI: Applicability of TOF-SIMS for the assessment of lipid composition of cell membrane structures

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Applicability of TOF-SIMS for the assessment of lipid composition of cell membrane structures

Content Provider	Springer Nature Link
Author	Gulin, A. A. Pavlyukov, M. S. Gusev, S. A. Malakhova, Yu. N. Buzin, A. I. Chvalun, S. N. Aldarov, K. G. Kliv, D. V. Gularyan, S. K. Nadtochenko, V. A.
Copyright Year	2017
Abstract	Langmuir–Blodgett lipid films and plasma membranes of glioma cells were analyzed using timeof-flight secondary ion mass spectrometry (TOF-SIMS). Bi$_{3}$ $^{+}$ primary ion beam was determined to be most efficient in various experimental setups. TOF-SIMS was shown to be applicable for a quantitative analysis of model lipid structures, as well as plasma membranes of glioma cells U87MG in vitro. A combination of atomic-force microscopy and scanning electron microscopy yielded the depth resolution of ~10–20 nm for cell surface scanning by primary ion beam.
Starting Page	144
Ending Page	150
Page Count	7
File Format	PDF
ISSN	19907478
Journal	Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Volume Number	11
Issue Number	2
e-ISSN	19907494
Language	English
Publisher	Pleiades Publishing
Publisher Date	2017-06-10
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	mass spectrometry TOF-SIMS quantitative analysis Langmuir–Blodgett films ion beam etching Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Biophysics

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