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Li/Na exchange and Li active transport in human lymphoid cells U937 cultured in lithium media

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Li/Na exchange and Li active transport in human lymphoid cells U937 cultured in lithium media

Content Provider	Springer Nature Link
Author	Yurinskaya, V. E. Moshkov, A. V. Goryachaya, T. S. Vereniv, A. A.
Copyright Year	2014
Abstract	Lithium transport across the cell membrane is interesting in the light of general cell physiology and because of its alteration during numerous human diseases. The mechanism of Li$^{+}$ transfer has been studied mainly in erythrocytes with a slow kinetics of ion exchange and therefore under the unbalanced ion distribution. Proliferating cultured cells with a rapid ion exchange have not been used practically in study of Li$^{+}$ transport. In the present paper, the kinetics of Li$^{+}$ uptake and exit, as well as its balanced distribution across the plasma membrane of U937 cells, were studied at minimal external Li$^{+}$ concentrations and after the whole replacement of external Na$^{+}$ for Li$^{+}$. It is found that a balanced Li$^{+}$ distribution attained at a high rate similar to that for Na$^{+}$ and Cl$^{−}$ and that Li$^{+}$/Na$^{+}$ discrimination under balanced ion distribution at 1–10 mM external Li$^{+}$ stays on 3 and drops to 1 following Na, K-ATPase pump blocking by ouabain. About 80% of the total Li$^{+}$ flux across the plasma membrane under the balanced Li$^{+}$ distribution at 5 mM external Li$^{+}$ accounts for the equivalent Li$^{+}$/Li$^{+}$ exchange. The majority of the Li$^{+}$ flux into the cell down the electrochemical gradient is a flux through channels and its small part may account for the NC and NKCC cotransport influxes. The downhill Li$^{+}$ influxes are balanced by the uphill Li$^{+}$ efflux involved in Li$^{+}$/Na$^{+}$ exchange. The Na$^{+}$ flux involved in the countertransport with the Li$^{+}$ accounts for about 0.5% of the total Na$^{+}$ flux across the plasma membrane. The study of Li$^{+}$ transport is an important approach to understanding the mechanism of the equivalent Li$^{+}$/Li$^{+}$/Na$^{+}$/Na$^{+}$ exchange, because no blockers of this mode of ion transfer are known and it cannot be revealed by electrophysiological methods. Cells cultured in the medium where Na$^{+}$ is replaced for Li$^{+}$ are recommended as an object for studying cells without the Na,K-ATPase pump and with very low intracellular Na$^{+}$ and K$^{+}$ concentration.
Starting Page	80
Ending Page	90
Page Count	11
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	8
Issue Number	1
e-ISSN	19905203
Language	English
Publisher	Springer US
Publisher Date	2014-02-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Li$^{+}$/Na$^{+}$ exchange lithium ion transport U937 cells Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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