NDLI: Morphofunctional changes of Jurkat T lymphoblasts upon short-term contact with a relief calcium phosphate surface

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Morphofunctional changes of Jurkat T lymphoblasts upon short-term contact with a relief calcium phosphate surface

Content Provider	Springer Nature Link
Author	Khlusov, I. A. Litviva, L. S. Shupletsova, V. V. Dunets, N. A. Khaziakhmatova, O. G. Yurova, K. A. Khlusova, M. Yu. Sharkeev, Yu. P.
Copyright Year	2017
Abstract	Human leukemic T lymphoblastoid cells (hereinafter, Jurkat T cells) have been used to model the morphofunctional reaction of T lymphocytes to 24-h in vitro contact with relief (roughness index Ra = 2.2–2.7 μm) pure titanium substrates (12 × 12 × 1 mm$^{3}$) covered by a calcium phosphate (CP) bilateral coating prepared by the microarc method. Jurkat T cells placed in plastic multiwall plates (2D control of culture growth), as well as cells in contact for 24 h with oxide (TiO$_{2}$) microarc coating on pure titanium substrate (3D control), served as comparison tests. From 27 to 98% of immortalized cells in the 2D control culture had the CD3$^{+}$CD4$^{+}$CD71$^{+}$CD45RA$^{+}$ immunophenotype and secreted IL-2, IL-4, IL-8, IL-10, and TNFα, but not IL-1b and IL-6. Other markers of cell activation, differentiation, maturation, and death (CD8, CD16, CD56, CD25, and CD95) were found in 0–2.5% of cells. IL-8 release elevated by the microtextured CP surface by 183 and 160% relative to 2D- and 3D-control cultures, respectively. The CD4/CD8 ratio dropped to 9: 1 (13: 1 and 82: 1 in 2D and 3D controls, respectively) due to CD4$^{+}$ cell depletion and an increase in CD8$^{+}$ cell number. The total cell number (TCN) in Jurkat T cell culture after 24-h contact with CP coating was decreased to 88% compared to 2D control (p < 0.04), which indicated cell division suppression. A drop in total cell number was accompanied with accelerated IL-8 secretion (r = −0.97, p < 0.00009). Low concentrations of IL-8 (pg/mL) induced apoptosis (r = 0.94, p < 0.0001). These results show that CP, rather than TiO$_{2}$, coating is a preferential surface material for endoprosthesis replacement and fracture osteosynthesis in patients suffering from hematological and bone malignancies.
Starting Page	59
Ending Page	64
Page Count	6
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	11
Issue Number	1
e-ISSN	19905203
Language	English
Publisher	Pleiades Publishing
Publisher Date	2017-02-16
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Jurkat T-cell line membrane markers cytokines apoptosis necrosis titanium substrates microarc calcium phosphate coating microtexture Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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