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Cell and Tissue Biology : Volume 6, Issue 2, April 2012

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Peculiarities of cytometrical methods of DNA content determination in the nucleus

Content Provider	Springer Nature Link
Author	Pichugin, Yu. G. Semiyav, K. A. Chernyshev, A. V. Palchikova, I. G. Omelyanchyuk, L. V. Maltsev, V. P.
Copyright Year	2012
Abstract	This work has proposed a new theoretical approach to analysis of histograms of DNA content, which are obtained by the method of flow cytometry, in cells of Drosophila melanogaster imaginal discs. The precision of measurements of the DNA amount in G$_{1}$ and G$_{2}$(M) phases has been shown to be limited by precision of instrument tuning of zero of the flow cytometer. Use of the calculative zero of the flow cytometer and of dividing cells as standards of the DNA content is able to increase severalfold the precision of the DNA measurements in nuclei of the species. Comparative analysis of errors of various methods of measurement of the DNA content in cell nuclei is also performed. For methods of flow fluorescent cytometry, confocal scanning, and cytophotometry of the Feulgen-stained nuclei, it has been shown that, at present, the mean square errors of the DNA content measurements are within the interval of values considered acceptable for biological studies (0.02 < CV < 0.06).
Starting Page	302
Ending Page	308
Page Count	7
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	6
Issue Number	3
e-ISSN	19905203
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2012-06-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	fluorescent flow cytometry DNA content cell cycle Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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