Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Shakhov, A. S. Dugina, V. B. Alieva, I. B. |
| Copyright Year | 2015 |
| Abstract | Endothelial cells tightly joined to each other line the interior surface of all vessels in living organisms and ensure the regulation of vascular permeability and exchange between the blood circulating in vessels and tissue fluids of those organs in which these vessels are located. In vitro, the endothelial cell monolayer retains the basic barrier function of the native endothelium. We use endothelial cells growing in vitro as a model system to study the interaction between cytoskeleton and adhesion structures in endotheliocytes. In the present paper, cultured human vein endothelial cells were used to quantify cytoskeleton alterations from cell spreading and formation of the first cell–cell contacts to confluent monolayer formation. The actin filament system in vein endothelial cells consists of a cortical network and stress fibers arranged parallel to the substrate. This cells express two isoforms of nonmuscle actin: β- and γ-cytoplasmic actins. Antibodies to β-actin stain actin filament bundles, whereas antibodies to γ-actin visualize cortical and lamellar networks. To evaluate the state of actin structures we analyzed their fluorescence intensity near the free edge and zone of cell–cell contacts. Fluorescence intensity of β-actin structures was higher in the areas of cell–cell contacts. Fluorescence of γ-actin was the highest at the leading edges of lamellae and the weakest in the stable edges of the cells with formed cell–cell contacts. The endothelial monolayer formation was accompanied by microtubule system alteration: the number of microtubules increased at the cell edge, and what was more, the quantity of microtubules in the area of the already-formed cell–cell contact always being higher than in the leading edge region. |
| Starting Page | 299 |
| Ending Page | 309 |
| Page Count | 11 |
| File Format | |
| ISSN | 1990519X |
| Journal | Cell and Tissue Biology |
| Volume Number | 9 |
| Issue Number | 4 |
| e-ISSN | 19905203 |
| Language | English |
| Publisher | Pleiades Publishing |
| Publisher Date | 2015-08-11 |
| Publisher Place | Moscow |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | endothelium cytoskeleton of endothelial cells γ-actin β-actin microtubules Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|