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Cytological analysis of the response of nucleolar RNA and RNA-binding proteins to oxidative stress in HeLa cells

Content Provider	Springer Nature Link
Author	Mirova, A. A. Barykina, N. V. Zatsepina, O. V.
Copyright Year	2014
Abstract	The organization and functional activity of the nucleoli in mammalian cells can alter in response to various stress stimuli. However, data on the nucleoli reaction to oxidative stress are limited. In this work, we studied localization of the nucleolar RNA and two RNA-binding proteins, the pre-rRNA processing factor fibrillarin and preribosome assembly factor nucleophosmin/B23, in HeLa cells exposed to 1 mM H$_{2}$O$_{2}$ up to 4 h. We have shown that, under these conditions, H$_{2}$O$_{2}$ does not induce cell death but inhibits rDNA transcription, reduces the total amount of RNA and 18S rRNA in nucleoli, and causes relocalization of fibrillarin and nucleophosmin/B23 to the nucleoplasm. Similar changes in fibrillarin localization have to date been described in mammalian cells only after HgCl$_{2}$ treatment. Redistribution of nucleophosmin/B23 observed in H$_{2}$O$_{2}$-treated cells has occurred in mammalian cells under inhibition of rDNA transcription and early rRNA processing. In general, this study shows that there is a high sensitivity of nucleoli in HeLa cells to acute oxidative stress at the cytological level.
Starting Page	461
Ending Page	472
Page Count	12
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	8
Issue Number	6
e-ISSN	19905203
Language	English
Publisher	Pleiades Publishing
Publisher Date	2014-12-17
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	fibrillarin FISH H$_{2}$O$_{2}$ nucleolus nucleophosmin/B23 oxidative stress pyronin Y rRNA Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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