NDLI: Mosaic expression of LacZ reporter gene controlled by 5′-regulatory sequences of alpha-S $

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Cell and Tissue Biology : Volume 3, Issue 4, August 2009

Cell and Tissue Biology : Volume 3, Issue 3, June 2009

Cell and Tissue Biology : Volume 3, Issue 2, April 2009

Cell and Tissue Biology : Volume 3, Issue 1, February 2009

Cell and Tissue Biology : Volume 2

Cell and Tissue Biology : Volume 1

Mosaic expression of LacZ reporter gene controlled by 5′-regulatory sequences of alpha-S $_{ 1 }$-Casein gene in transgenic mice

Content Provider	Springer Nature Link
Author	Serova, I. A. Andreeva, L. E. Khaidarova, N. V. Dias, L. P. B. Dvoryanchikov, G. A. Burkov, I. A. Baginskaya, N. V.
Copyright Year	2009
Abstract	The phenomenon of mosaic expression at the cellular level is frequently observed in tissues and organs of transgenic animals. The report concerns mosaicism in the progeny of five transgenic mouse founders carrying the LacZ reporter gene under the control of 5′-regulatory sequences of bovine and goat alpha-S $_{ 1 }$-casein genes of various sizes. Cells positive for β-galactosidase E. coli activity were detected in lactating mammary glands of all transgenic females; however, the distribution of positive cells within the mammary glands was variable. We observed two types of mosaics, i.e., lobular (clonal) variegation when most or all lobular cells were positive for β-galactosidase and stochastic mosaicism when only single β-galactosidase positive cells were scattered within mammary glands. It is suggested that the stochastic type of mosaicism is realized in cells at the terminal stage of the differentiation of lactating glands, whereas the lobular one is developed from proliferating precursors capable of forming a whole lobule. The ectopic expression of the reporter gene was detected in the mandibular salivary gland in the offspring of two of the five founders No16 and No37, as well as in ovary follicles at the atrezia stage in the progeny of one of these founders. The low level of ectopic expression means that the 5′-flanked regulatory sequences of alpha-S $_{ 1 }$-casein gene of various lengths used in the constructs ensure the reliable tissue-specific expression of the reporter gene.
Starting Page	409
Ending Page	416
Page Count	8
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	3
Issue Number	5
e-ISSN	19905203
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-11-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	transgenesis cell mosaicism LacZ reporter gene Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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