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| Content Provider | Springer Nature Link |
|---|---|
| Author | Antova, L. V. Matveeva, V. G. Cherva, M. N. Velikava, E. A. Ponasenko, A. V. Golovkin, A. S. |
| Copyright Year | 2014 |
| Abstract | In this study, we examined the impact of 3-day hypoxia of different degrees on the viability, proliferation, and secretory activity of endothelial cells from human umbilical vein (HUVEC). A gas mixture of three components was used (%): 1) 10 O$_{2}$, 5 CO$_{2}$, and 85 Ar; 2) 5 O$_{2}$, 5 CO$_{2}$, and 80 Ar; and 3) 1 O$_{2}$, 5 CO$_{2}$, and 94 Ar. Cells cultivated in a CO$_{2}$ incubator in atmospheric oxygen (21% O$_{2}$) served as control. It was found that 3-day HUVEC cultivation at 1% O$_{2}$ increased NO synthesis; enhanced secretion of endothelin-1, IL-6, IL-8, TNF-alpha, sVCAM-1, sE-cadherin, sE-selectin, VEGF-A, and bFGF; and inhibited proliferation. HUVEC cultivated under 10% O$_{2}$ and 5% O$_{2}$ exhibited the lowest level of basal secretion of these substances and increased proliferative activity. These cells cultivated under conditions of atmospheric oxygen for 3 days displayed activated secretion of NO, IL-6, IL-8, and von Willebrand factor; the activation was higher than at 10% O$_{2}$ and 5% CO$_{2}$. Thus, the gaseous medium with reduced oxygen content (5%) is a more physiological condition for HUVEC cultivation. An increase in the amount of oxygen up to the atmospheric level causes endotheliocyte activation; the cells exhibit the features of endothelial dysfunction. Oxygen content reduced to 1% induces endothelial dysfunction and reduced proliferative potential. |
| Starting Page | 204 |
| Ending Page | 212 |
| Page Count | 9 |
| File Format | |
| ISSN | 1990519X |
| Journal | Cell and Tissue Biology |
| Volume Number | 8 |
| Issue Number | 3 |
| e-ISSN | 19905203 |
| Language | English |
| Publisher | Pleiades Publishing |
| Publisher Date | 2014-06-12 |
| Publisher Place | Moscow |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | hypoxia endothelial cells proliferation dysfunction cytokines Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology |
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