NDLI: The isolation and identification of salt-responsive novel microRNAs from Salicornia brachiata, an extreme halophyte

Please wait, while we are loading the content...

The isolation and identification of salt-responsive novel microRNAs from Salicornia brachiata, an extreme halophyte

Content Provider	Springer Nature Link
Author	Singh, Dinkar Jha, Bhavanath
Copyright Year	2014
Abstract	The small, non-coding RNA species (20–24 nucleotides), including microRNAs (miRNAs) and small interfering RNAs, are key regulators of gene expression in eukaryotic cells. Mostly, miRNAs have been identified from glycophytes under different abiotic stresses. The isolation and identification of miRNAs from halophytes are challenging due to the presence of high salt concentrations. In this study, small RNA cDNA libraries were constructed using an improved method to identify salt-responsive novel miRNAs from Salicornia brachiata. A total of 159 sequences were cloned and analysed. There was no adapter contamination and low-abundance small RNAs were amplified efficiently. Twelve putative miRNAs/pre-miRNAs and a small RNA were identified and confirmed by northern blot. Among the putative miRNAs, nine are novel and three belong to the conserved miRNA families (MIR169g, MIR1433, MIR138b). Northern hybridisation and real-time PCR showed that nine miRNAs (seven novel and two conserved) and one small RNA were highly expressed at 2.0 M NaCl, two novel miRNAs at 1.5 M NaCl, and one conserved miRNA at 1.0 M NaCl. Furthermore, 67 putative target genes of the isolated miRNA were predicted on the basis of sequence homology, of which more than 50 % are associated with abiotic stresses. The target genes of miRNA 169g (Sb-miRNA10), and Sb-miRNA7 (NF-YA transcription factor and cytochrome P$_{450}$-like TATA box binding protein respectively) were analysed by real-time PCR. The study of miRNAs from an extreme halophyte broadens our understanding of the important role played by miRNAs in plant abiotic stress.
Starting Page	325
Ending Page	336
Page Count	12
File Format	PDF
ISSN	18635466
Journal	Plant Biotechnology Reports
Volume Number	8
Issue Number	4
e-ISSN	18635474
Language	English
Publisher	Springer Japan
Publisher Date	2014-05-29
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Low molecular weight RNAs Small RNAs cloning Salt-stress miRNAs expression Salicornia brachiata Plant Sciences Cell Biology Plant Biochemistry Biotechnology Agriculture
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

SbDREB2A, an A-2 type DREB transcription factor from extreme halophyte Salicornia brachiata confers abiotic stress tolerance in Escherichia coli

The SbASR-1 Gene Cloned from an Extreme Halophyte Salicornia brachiata Enhances Salt Tolerance in Transgenic Tobacco

The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na+loading in xylem and confers salt tolerance in transgenic tobacco

Physiological and Biochemical Responses Reveal the Drought Tolerance Efficacy of the Halophyte Salicornia brachiata

Cloning and characterization of the Salicornia brachiata Na$^{+}$/H$^{+}$ antiporter gene SbNHX1 and its expression by abiotic stress

Antioxidative Defense Potential to Salinity in the Euhalophyte Salicornia brachiata

A Dehydration-Responsive Element Binding (DREB) Transcription Factor from the Succulent Halophyte Salicornia brachiata Enhances Abiotic Stress Tolerance in Transgenic Tobacco

Cloning and characterization of novel salt responsive genes SbRPC5 and SbCPN10 like from Salicornia brachiata

Cloning and transcript analysis of type 2 metallothionein gene (SbMT-2) from extreme halophyte Salicornia brachiata and its heterologous expression in E. coli.

The isolation and identification of salt-responsive novel microRNAs from Salicornia brachiata, an extreme halophyte

Similar Documents

SbDREB2A, an A-2 type DREB transcription factor from extreme halophyte Salicornia brachiata confers abiotic stress tolerance in Escherichia coli

The SbASR-1 Gene Cloned from an Extreme Halophyte Salicornia brachiata Enhances Salt Tolerance in Transgenic Tobacco

The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na+loading in xylem and confers salt tolerance in transgenic tobacco

Physiological and Biochemical Responses Reveal the Drought Tolerance Efficacy of the Halophyte Salicornia brachiata

Cloning and characterization of the Salicornia brachiata Na$^{+}$/H$^{+}$ antiporter gene SbNHX1 and its expression by abiotic stress

Antioxidative Defense Potential to Salinity in the Euhalophyte Salicornia brachiata

A Dehydration-Responsive Element Binding (DREB) Transcription Factor from the Succulent Halophyte Salicornia brachiata Enhances Abiotic Stress Tolerance in Transgenic Tobacco

Cloning and characterization of novel salt responsive genes SbRPC5 and SbCPN10 like from Salicornia brachiata

Cloning and transcript analysis of type 2 metallothionein gene (SbMT-2) from extreme halophyte Salicornia brachiata and its heterologous expression in E. coli.

The isolation and identification of salt-responsive novel microRNAs from Salicornia brachiata, an extreme halophyte