NDLI: Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis

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Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis

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Korean Journal of Chemical Engineering : Volume 26, Issue 2, March 2009

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Korean Journal of Chemical Engineering : Volume 23

Korean Journal of Chemical Engineering : Volume 22

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Korean Journal of Chemical Engineering : Volume 17

Korean Journal of Chemical Engineering : Volume 16

Korean Journal of Chemical Engineering : Volume 15

Korean Journal of Chemical Engineering : Volume 14

Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis

Content Provider	Springer Nature Link
Author	Kim, Youngo Lee, Taeho Park, Taejoo Park, Heesu Lee, Sanghyup
Copyright Year	2009
Abstract	This study was conducted by combining fluorescence in situ hybridization (FISH) performed on 16S rRNA and polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) with 16S CTOs primers to characterize the nitrifying microbial communities in biofilm processes, which were tested to retrofit the S municipal wastewater treatment plant in Busan, Korea. Four aerophilic biofilm reactors were operated with hydraulic retention times of 2 to 8 h and biofilms were grown on ceramic media. The same low COD/ NH 4 + -N ratio (100 mg/L of COD over 40 mg/L of NH 4 + -N) with the S plant was used. The average relative population ratios of Nitrosomonas spp. to ammonia oxidizing bacteria (AOB) as measured by specific FISH probes (%/Nso190) were 75.0%, 80.0%, 73.0% and 73.5%, respectively, while those of Nitrosospira spp. to AOB were 21.0%, 14.7%, 24.6% and 24.1% after 180 days of operation. The microbial composition of Nitrobacter spp. detected by using a Nit3 probe was below 10% in each reactor. In contrast, Nitrospira genus detected with an Ntspa662 probe was around 20%. When CTOs primer was applied in PCR-DGGE analysis to define the nitrifying bacteria, the bands of group B in the R-1 reactor with the highest hydraulic retention time (HRT) had the strongest light intensity compared with two other reactors with lower HRTs after day 64. The bands of the groups were responsible for nitrification with the major dominant population in each reactor depending on the change of ammonia removal rate. These results would directly lead to an understanding of the reactor performance in relation to the ammonia removal, when conventional municipal wastewater treatment plants are retrofitted or upgraded to biological nitrogen removal processes using biofilm.
Starting Page	685
Ending Page	690
Page Count	6
File Format	PDF
ISSN	02561115
Journal	Korean Journal of Chemical Engineering
Volume Number	26
Issue Number	3
e-ISSN	19757220
Language	English
Publisher	Springer US
Publisher Date	2009-05-20
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aerophilic Biofilm Ammonia Oxidizing Bacteria FISH Nitrification PCR-DGGE Biotechnology Materials Science Catalysis Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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Nitrifying bacterial communities and its activities in aerobic biofilm reactors under different temperature conditions

Comparison of ammonia-oxidizing bacterial community structure in membrane-assisted bioreactors using PCR-DGGE and FISH.

16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Community survey of ammonia-oxidizing bacteria in full-scale activated sludge processes with different solids retention time

Change in the community structure of ammonia-oxidizing bacteria in activated sludge during selective incubation for MPN determination

Ammonia oxidizing bacteria community in activated sludge monitored by denaturing gradient gel electrophoresis (DGGE)

16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting.

Population dynamics of ammonia-oxidizing bacteria in an aerated submerged biofilm reactor for micropolluted raw water pretreatment

16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis

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