NDLI: Expression characteristics of Odf3 in gonad are different between Chlamys farreri and vertebrates

Content Provider	Springer Nature Link
Author	Ma, Xiaoshi Liang, Shaoshuai Yang, Dandan Ji, Aichang Ma, Xiaoru Zhang, Zhifeng
Copyright Year	2017
Abstract	Outer dense fibers are specific subcellular components of mammal sperm flagella, apart from the axoneme and its associated proteins. As one of the outer dense fibers components, ODF3 is essential for the formation of mammal sperm flagella. In the present study, we isolated the Odf3 gene, 1033 bp in length, from Zhikong scallop Chlamys farreri, which was named as Cf-Odf3. It was predicted that Cf-Odf3 encodes a protein of 240 amino acids which contains 5 discontinuous Pro-Gly-Pro repeats. The Cf-Odf3 transcripts were detected in both testis and ovary of C. farreri at various stages through qRT-PCR. The Cf-ODF3 protein synthesized by prokaryotes was purified using Ni$^{2+}$-NTA affinity chromatography and used to produce its polyclonal antibody. The antibody product was effective and specific as was examined by Western blotting. The Cf-Odf3 transcript and Cf-ODF3 protein were located in intragonadal somatic cells (ISCs), spermatogonia and spermatocytes of C. farreri testes, while no transcription of Cf-Odf3 and synthesis of Cf-ODF3 were detected in spermatids and spermatozoa. The location of Odf3 expression is different from that of vertebrates where ODF3 is synthesized specifically in spermatids and sperm flagella. Moreover, the Cf-Odf3 expression was also detected in ISCs, oogonia and oocytes of the scallop ovaries. Our finding revealed a different characteristic of Odf3 expression between scallop and vertebrates, which implied that Cf-Odf3 played a role in the gametogenesis of C. farreri.
Starting Page	121
Ending Page	128
Page Count	8
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	16
Issue Number	1
e-ISSN	19935021
Language	English
Publisher	Science Press
Publisher Date	2017-01-08
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Odf3 germ cell gametogenesis gonad Chlamys farreri Oceanography Meteorology
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization, expression and function analysis of DAX1 gene of scallop (Chlamys farreri jones and preston 1904) during its gametogenesis

Sexually dimorphic expression of foxl2 during gametogenesis in scallop Chlamys farreri, conserved with vertebrates

Expression pattern of phb2 and its potential function in spermatogenesis of scallop (Chlamys farreri)

Expression pattern of Chlamys farreri sox2 in eggs, embryos and larvae of various stages

Association of myostatin variants with growth traits of Zhikong scallop (Chlamys farreri)

Mapping toll-like receptor signaling pathway genes of Zhikong scallop (Chlamys farreri) with FISH

Changes in global DNA methylation intensity and DNMT1 transcription during the aging process of scallop Chlamys farreri

Development of 101 novel EST-derived single nucleotide polymorphism markers for Zhikong scallop (Chlamys farreri)

Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)

Expression characteristics of Odf3 in gonad are different between Chlamys farreri and vertebrates

Similar Documents

Characterization, expression and function analysis of DAX1 gene of scallop (Chlamys farreri jones and preston 1904) during its gametogenesis

Sexually dimorphic expression of foxl2 during gametogenesis in scallop Chlamys farreri, conserved with vertebrates

Expression pattern of phb2 and its potential function in spermatogenesis of scallop (Chlamys farreri)

Expression pattern of Chlamys farreri sox2 in eggs, embryos and larvae of various stages

Association of myostatin variants with growth traits of Zhikong scallop (Chlamys farreri)

Mapping toll-like receptor signaling pathway genes of Zhikong scallop (Chlamys farreri) with FISH

Changes in global DNA methylation intensity and DNMT1 transcription during the aging process of scallop Chlamys farreri

Development of 101 novel EST-derived single nucleotide polymorphism markers for Zhikong scallop (Chlamys farreri)

Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)

Expression characteristics of Odf3 in gonad are different between Chlamys farreri and vertebrates