NDLI: Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites

Content Provider	Springer Nature Link
Author	Juan, Chen Tianjiao, Zhu Dehai, Li Chengbin, Cui Yuchun, Fang Hongbing, Liu Peipei, Liu Qianqun, Gu Weiming, Zhu
Copyright Year	2006
Abstract	To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of spongeGelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper dise assaying. Two clones displayed the antibacterial activity againstMicrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the hostE. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.
Starting Page	119
Ending Page	122
Page Count	4
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	5
Issue Number	2
e-ISSN	19935021
Language	English
Publisher	Science Press
Publisher Date	2006-01-01
Publisher Place	Beijing
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	metagenomic DNA library sponge symbionts metabolite bioactivity Oceanography
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

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Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites