NDLI: Floating Escherichia coli by expressing cyanobacterial gas vesicle genes

Content Provider	Springer Nature Link
Author	Wang, Tianhe Kang, Li Li, Jiaheng Wu, Wenjie Zhang, Peiran Gong, Minghao Lai, Weihong Zhang, Chunyan Chang, Lei Peng, Yong Yang, Zhongzhou Li, Lian Bao, Yingying Xu, Haowen Zhang, Xiaohua Sui, Zhenghong Yang, Guanpin Wang, Xianghong
Copyright Year	2014
Abstract	Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20Ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvpA and gvpC20Ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvpA and gvpC20Ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their cells.
Starting Page	84
Ending Page	88
Page Count	5
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	14
Issue Number	1
e-ISSN	19935021
Language	English
Publisher	Science Press
Publisher Date	2014-11-12
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	gas vesicle gene cluster gas vesicle gene buoyancy Oceanography Meteorology
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

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