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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Tong Xie, Xing Hu, Hongying Song, Yudong Wu, Qianyuan Zong, Zusheng |
| Copyright Year | 2008 |
| Abstract | Cryptosporidium and Giardia are two typical species of pathogenic protozoans that seriously endanger water quality. Previous works indicated that detection of Cryptosporidium and Giardia with modified United States Environmental Protection Agency (USEPA) method-1623 using a membrane filtration-elution for sample concentration attained better recovery and lower cost compared to the USEPA method-1623. Several improvements of membrane filtration-elution step as well as immunomagnetic separation (IMS) steps were investigated and an optimized method for detection of Cryptosporidium and Giardia in wastewater reclamation system was recommended in this paper. The experimental results show that an overnight soak of the membrane after scraping and vortex agitation before elution could enhance and stabilize the recovery. Increasing turbidity to 4 NTU by adding kaolin clay before filtration could effectively improve the recovery of low-turbidity water. Washing the concentrate after centrifugation and twice acid dissociation both reduced the impact of water quality to protozoan recovery. Protozoans in different water samples were determined by this optimized method, and the recovery of Cryptosporidium and Giardia were above 70% and 80% respectively, much higher than the acceptance of method-1623. |
| Starting Page | 380 |
| Ending Page | 384 |
| Page Count | 5 |
| File Format | |
| ISSN | 16737415 |
| Journal | Frontiers of Environmental Science & Engineering in China |
| Volume Number | 2 |
| Issue Number | 3 |
| e-ISSN | 16737520 |
| Language | English |
| Publisher | SP Higher Education Press |
| Publisher Date | 2008-08-06 |
| Publisher Institution | Chinese Universities |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | concentration Cryptosporidium Giardia immunomagnetic separation (IMS) wastewater reuse Environment |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Science |
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