NDLI: Effect of microwave irradiation on secondary structure of α-amylase by circular dichroism

Content Provider	Springer Nature Link
Author	Zhang, Xiao yun Qin, Wen qing Tian, Xue da Huang, Meng
Copyright Year	2011
Abstract	Based on the starch hydrolysis reaction accelerated by microwave irradiation with α-amylase, the circular dichroism (CD) and secondary structure changes of α-amylase under the condition of microwave irradiation and water bath were studied by circular dichroism spectra. The results showed that, both the peak heights (at λ=193 nm) of the CD spectra of the samples treated by microwave irradiation and water bath reduced. The reduced rate by microwave irradiation ranged from 140% to 220%, while the reduced rate by water bath ranged from 60% to 140%. The peak of the sample treated by microwave irradiation for 60 min disappeared at λ=193 nm, while the sample showed a wake peak by water bath. The peak position by microwave irradiation emerged a blue shift in the range of 5–8 nm at λ=204 nm and λ=220 nm, while it emerged in the range of 3–5 nm by water bath. With time going on, the microwave irradiation and water bath have prompted the secondary structure of α-helix, β-sheet, β-turn and the mutual transformations of random coil, but the trends were different.
Starting Page	1029
Ending Page	1033
Page Count	5
File Format	PDF
ISSN	10059784
Journal	Journal of Central South University of Technology
Volume Number	18
Issue Number	4
e-ISSN	19930666
Language	English
Publisher	Central South University
Publisher Date	2011-07-10
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	microwave irradiation α-amylase structure circular dichroism spectra Metallic Materials Engineering
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Materials Science Mechanical Engineering

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