NDLI: Validation of a Method for Quantitation of Soybean Lectin in Commercial Varieties

Content Provider	Springer Nature Link
Author	Breeze, Matthew L. Leyva Guerrero, Elisa Yeaman, Grant R. Dudin, Yelena Akel, Ryan Brune, Phil Claussen, Fred Dharmasri, Cecil Golbach, Jenny Guo, Rong Maxwell, Carl Privalle, Laura Rogers, Hilary Liu, Kai Shan, Guomin Yarnall, Michele Thiede, Denise Gillikin, Nancy
Copyright Year	2015
Abstract	Soybean agglutinin (SBA) protein, also known as soybean lectin, is regarded as an anti-nutrient due to its negative effect on the ability of monogastric animals to gain weight following consumption of raw soybean seed. Historically, SBA has been measured using a time-consuming and cumbersome hemagglutination procedure. The objective of our research was to obtain a validated methodology that is precise and accurate in the measurement of SBA while allowing minimally equipped laboratories to effectively conduct the analysis, thus our focus was on evaluating an existing commercially available ELISA, an enzyme-linked-lectin-assay (ELLA), and a hybrid ELISA/ELLA. A new ELLA technique that can detect and quantify lectins was chosen and modified specifically for the quantitation of SBA in soybean seed. The proposed ELLA methodology is similar to a standard sandwich ELISA, and uses polyacrylamide-linked N-acetylgalactosamine (Gal–NAc–PAA) for a capture phase and the biotinylated version (Gal–NAc–PAA–Biotin) for detection. Based upon the validation data, the ELLA method can precisely and accurately determine soybean lectin levels in soybean seed. The validated ELLA method was used to quantify SBA in nine commercial soybean varieties introduced between 1972 and 2008 and demonstrated that the natural variability of SBA is subject to the effects of genotype and environment.
Starting Page	1085
Ending Page	1092
Page Count	8
File Format	PDF
ISSN	0003021X
Journal	Journal of the American Oil Chemists' Society
Volume Number	92
Issue Number	8
e-ISSN	15589331
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Soybean Lectin Agglutinin Composition ELLA SBA Enzyme linked Industrial Chemistry/Chemical Engineering Biomaterials Agriculture Food Science Biotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Chemical Engineering

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