NDLI: Oil extraction from microalga Nannochloropsis sp. with isopropyl alcohol

Content Provider	Springer Nature Link
Author	Yao, Linxing Gerde, Jose A. Wang, Tong
Copyright Year	2012
Abstract	An efficient process for fractioning microalgae oil and non-lipid biomass was developed. Isopropyl alcohol (IPA) was used to extract oil from Nannochloropsis sp. at 80 °C, leaving the majority of non-lipid biomass in the solid fraction. The effectiveness of extraction with or without a dewatering pretreatment (DW) was compared. Effects of dewatering time and solvent ratio, IPA concentration, IPA refluxing time, and sonication pretreatment on the oil and biomass yields were studied. The dewatering conditions with a high water-to-alcohol ratio (W/A = 2:1) and mild mixing (1 min gentle shaking) had 14 % less oil loss in the DW fraction than that with a low water-to-alcohol ratio (W/A = 1:1) and vigorous mixing (30 min and 300 rpm mixing). Sonication resulted in 14–26 % more oil loss in the DW fraction when compared to intact cell treatment. Without dewatering, 85 % of the total oil from intact cells was extracted by a single extraction using 70 % (wt) IPA aqueous solution. The 88 and 95 % IPA treatments extracted similar percentages of oil to that of the 70 % IPA, but used two- and fivefold more solvent. The amount of oil extracted from broken cells increased with increasing IPA concentrations. An effective extraction can be completed in 30 min. On a 100-g (wet matter) scale, the 70 % IPA achieved 92 % oil yield and 93 % non-lipid biomass yield.
Starting Page	2279
Ending Page	2287
Page Count	9
File Format	PDF
ISSN	0003021X
Journal	Journal of the American Oil Chemists' Society
Volume Number	89
Issue Number	12
e-ISSN	15589331
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dewatering Isopropyl alcohol Microalgae Oil extraction Sonication Agriculture Food Science Biomaterials Industrial Chemistry/Chemical Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Chemical Engineering

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