NDLI: Quantification of the Molecular Species of TAG and DAG in Lesquerella (Physaria fendleri) Oil by HPLC and MS

Content Provider	Springer Nature Link
Author	Lin, Jiann Tsyh Chen, Grace Q.
Copyright Year	2014
Abstract	Ten diacylglycerols (DAG) and 74 triacylglycerols (TAG) in the seed oil of Physaria fendleri were recently identified by high-performance liquid chromatography (HPLC) and mass spectrometry (MS). These acylglycerols (AG) were quantified by HPLC with evaporative light scattering detector and electrospray ionization mass spectrometry of the lithium adducts of the AG in the HPLC fractions of lesquerella oil. The MS$^{1}$ ion signal intensities of molecular ions [M + Li]$^{+}$ in HPLC fractions of an HPLC peak were used to estimate the ratios of AG in the HPLC peak. The ratios of TAG with the same mass in HPLC fractions were estimated by the ratios of the sums of MS$^{2}$ ion signal intensities from the neutral loss of the three fatty acids [M + Li − FA]$^{+}$. The ratio of DAG with the same mass were estimated by the ratio of the sums of two MS$^{2}$ ion signal intensities [M + Li − FA]$^{+}$ and [FA + Li]$^{+}$ from the two different FA of a DAG. We have estimated the contents of ten molecular species of DAG and 74 molecular species of TAG in P. fendleri oil using this new method. The content of ten DAG combined was about 1 % and 74 TAG was about 98 %. The contents of DAG in decreasing order were: LsLs (0.25 %), LsLn (0.25 %), LsO (0.24 %), and LsL (0.11 %); and the contents of TAG in decreasing order were: LsLsO (31.3 %), LsLsLn (24.9 %), LsLsL (15.8 %), LsL-OH20:2 (4.3 %), LsO-OH20:2 (2.8 %), and LsLn-OH20:2 (2.5 %).
Starting Page	1417
Ending Page	1424
Page Count	8
File Format	PDF
ISSN	0003021X
Journal	Journal of the American Oil Chemists' Society
Volume Number	91
Issue Number	8
e-ISSN	15589331
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-06-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Quantification Triacylglycerols Diacylglycerols Lesquerella oil Physaria fendleri HPLC ELSD MS Industrial Chemistry/Chemical Engineering Biomaterials Agriculture Food Science Biotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Chemical Engineering

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Identification of TAG and DAG and their FA Constituents in Lesquerella (Physaria fendleri) Oil by HPLC and MS

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