NDLI: Fetal Bovine Serum Concentration Affects Δ$^{9}$ Desaturase Activity of Trypanosoma cruzi

Content Provider	Springer Nature Link
Author	Villasuso, Ana L. Romero, Patricio Woelke, Mariela Moya, Patricia Machado, Estela García de Lema, Mirta
Copyright Year	2010
Abstract	Fetal bovine serum (FBS) is an important factor in the culture of Trypanosoma cruzi, since this parasite obtains and metabolizes fatty acids (FAs) from the culture medium, and changes in FBS concentration reduce the degree of unsaturation of FAs in phosphoinositides. When T. cruzi epimastigotes were cultured with 5% instead of 10% FBS, and stearic acid was used as the substrate, ∆$^{9}$ desaturase activity decreased by 50%. Apparent K $_{m}$ and V $_{m}$ values for stearic acid, determined from Lineaweaver–Burk plots, were 2 μM and 219 pmol/min/mg of protein, respectively. In studies of the requirement for reduced pyridine nucleotide, ∆$^{9}$ desaturase activity reached a maximum with 8 μM NADH and then remained constant; the apparent K $_{m}$ and V $_{m}$ were 4.3 μM and 46.8 pmol/min/mg of protein, respectively. The effect of FBS was observed only for ∆$^{9}$ desaturase activity; ∆$^{12}$ desaturase activity was not affected. The results suggest that decreased FBS in culture medium is a signal that modulates ∆$^{9}$ desaturase activity in T. cruzi epimastigotes.
Starting Page	275
Ending Page	283
Page Count	9
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	45
Issue Number	3
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-02-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Trypanosoma cruzi ∆$^{9}$ Desaturase ∆$^{12}$ Desaturase Fetal bovine serum Microbial Genetics and Genomics Medicinal Chemistry Nutrition Medical Biochemistry Neurochemistry Lipidology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

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