NDLI: The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

Please wait, while we are loading the content...

Gut Microbial Metabolites of Polyunsaturated Fatty Acids Correlate with Specific Fecal Bacteria and Serum Markers of Metabolic Syndrome in Obese Women

Lipids : Volume 49, Issue 3, March 2014

Lipids : Volume 49, Issue 2, February 2014

Lipids : Volume 49, Issue 1, January 2014

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

Content Provider	Springer Nature Link
Author	Furmanek, Tomasz Demski, Kamil Banaś, Walentyna Haslam, Richard Napier, Jonathan Stymne, Sten Banaś, Antoni
Copyright Year	2014
Abstract	The triacylglycerol of Crambe abyssinica seeds consist of 95 % very long chain (>18 carbon) fatty acids (86 % erucic acid; 22:1$^{∆13}$) in the sn-1 and sn-3 positions. This would suggest that C. abyssinica triacylglycerols are not formed by the action of the phospholipid:diacylglycerol acyltransferase (PDAT), but are rather the results of acyl-CoA:diacylglycerol acyltransferase (DGAT) activity. However, measurements of PDAT and DGAT activities in microsomal membranes showed that C. abyssinica has significant PDAT activity, corresponding to about 10 % of the DGAT activity during periods of rapid seed oil accumulation. The specific activity of DGAT for erucoyl-CoA had doubled at 19 days after flowering compared to earlier developmental stages, and was, at that stage, the preferred acyl donor, whereas the activities for 16:0-CoA and 18:1-CoA remained constant. This indicates that an expression of an isoform of DGAT with high specificity for erucoyl-CoA is induced at the onset of rapid erucic acid and oil accumulation in the C. abyssinica seeds. Analysis of the composition of the acyl-CoA pool during different stages of seed development showed that the percentage of erucoyl groups in acyl-CoA was much higher than in complex lipids at all stages of seed development except in the desiccation phase. These results are in accordance with published results showing that the rate limiting step in erucic acid accumulation in C. abyssinica oil is the utilization of erucoyl-CoA by the acyltransferases in the glycerol-3-phosphate pathway.
Starting Page	327
Ending Page	333
Page Count	7
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	49
Issue Number	4
e-ISSN	15589307
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Crambe PDAT DGAT Microsomal preparation Triacylglycerol Erucic acid Lipids Lipidology Neurochemistry Medical Biochemistry Nutrition Medicinal Chemistry Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

Two Clades of Type-1 Brassica napus Diacylglycerol Acyltransferase Exhibit Differences in Acyl-CoA Preference

RNAi Targeting Putative Genes in Phosphatidylcholine Turnover Results in Significant Change in Fatty Acid Composition in Crambe abyssinica Seed Oil

Activities of acyl-CoA:diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase (PDAT) in microsomal preparations of developing sunflower and safflower seeds

A Novel Assay of DGAT Activity Based on High Temperature GC/MS of Triacylglycerol

Simple Methods to Detect Triacylglycerol Biosynthesis in a Yeast-Based Recombinant System

Acyl-Trafficking During Plant Oil Accumulation

The Acyl CoenzymeA:Monoacylglycerol Acyltransferase 3 (MGAT3) Gene is a Pseudogene in Mice but Encodes a Functional Enzyme in Rats

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

Similar Documents

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil

Two Clades of Type-1 Brassica napus Diacylglycerol Acyltransferase Exhibit Differences in Acyl-CoA Preference

RNAi Targeting Putative Genes in Phosphatidylcholine Turnover Results in Significant Change in Fatty Acid Composition in Crambe abyssinica Seed Oil

Activities of acyl-CoA:diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase (PDAT) in microsomal preparations of developing sunflower and safflower seeds

A Novel Assay of DGAT Activity Based on High Temperature GC/MS of Triacylglycerol

Simple Methods to Detect Triacylglycerol Biosynthesis in a Yeast-Based Recombinant System

Acyl-Trafficking During Plant Oil Accumulation

The Acyl CoenzymeA:Monoacylglycerol Acyltransferase 3 (MGAT3) Gene is a Pseudogene in Mice but Encodes a Functional Enzyme in Rats

The Utilization of the Acyl-CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid-Rich Triacylglycerols in Crambe Seed Oil