NDLI: Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures

Content Provider	Springer Nature Link
Author	Źróbek Sokolnik, Anna Asard, Han Górska Koplińska, Kamilla Górecki, Ryszard J.
Copyright Year	2008
Abstract	Generation of reactive oxygen species (ROS) constitutes an important first reaction under many stress conditions in plants. We demonstrate that Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures, generate superoxide radical and hydrogen peroxide upon treatment with cadmium and zinc. Addition of catalase and N,N-diethyldithiocarbamate (DDC) decreased the level of H$_{2}$O$_{2}$, whereas superoxide dismutase (SOD) induced a slight increase of the H$_{2}$O$_{2}$ production. The effects of catalase, DDC and SOD on the heavy metal-induced ROS production indicate that it occurs outside of the cells, and that at least part of the hydrogen peroxide is produced by dismutation of the superoxide radical (O$_{2}$ $^{·−}$). The effect of pretreatment of the cell cultures with commonly used mammalian NADPH oxidase inhibitors was also tested. Strong inhibitions of cadmium and zinc-mediated ROS production were obtained with the flavoprotein inhibitors—diphenylene iodonium (DPI) and quinacrine and with an inhibitor of b-type cytochromes—imidazol. Membrane permeable-N-ethyl maleimide (NEM) and iodoacetate, and membrane non-permeable thiol reagents—para-chloromercuribenzoic acid (pCMBS) also inhibited the ROS production. These results suggested that the enzyme responsible for cadmium and zinc-induced ROS production in tobacco cells contains a flavocytochrome. They also show the importance of intra- and extracellular thiol groups in the observed stress reaction. The induction of ROS production with heavy metals showed properties comparable to the elicitor-induced oxidative burst in other plant cells.
Starting Page	43
Ending Page	49
Page Count	7
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	31
Issue Number	1
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Heavy metals Nicotiana tabacum Bright Yellow 2 Oxidative burst Oxidative stress Reactive oxygen species Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The cantharidin-induced oxidative burst in tobacco BY-2 cell suspension cultures

Copper-mediated oxidative burst in Nicotiana tabacum L. cv. Bright Yellow 2 cell suspension cultures

Endogenous hydrogen sulfide regulated by calcium is involved in thermotolerance in tobacco Nicotiana tabacum L. suspension cell cultures

Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

H2O2 Involvement in Polyamine-Induced Cell Death in Tobacco Leaf Discs

Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf disks exposed to cadmium

Integrative approach gives new insights into combined Cd/Cu exposure in tobacco

Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures

Similar Documents

The cantharidin-induced oxidative burst in tobacco BY-2 cell suspension cultures

Copper-mediated oxidative burst in Nicotiana tabacum L. cv. Bright Yellow 2 cell suspension cultures

Endogenous hydrogen sulfide regulated by calcium is involved in thermotolerance in tobacco Nicotiana tabacum L. suspension cell cultures

Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

Regulation of elicitin-induced ethylene production in suspension-cultured tobacco BY-2 cells

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

H2O2 Involvement in Polyamine-Induced Cell Death in Tobacco Leaf Discs

Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf disks exposed to cadmium

Integrative approach gives new insights into combined Cd/Cu exposure in tobacco

Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures