NDLI: Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105

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Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105

Content Provider	Springer Nature Link
Author	Szczerbakowa, Anna Bołtowicz, Danuta Lebecka, Renata Radomski, Paweł Wielgat, Bernard
Copyright Year	2005
Abstract	The morphological, cytological and molecular analyses of the plants regenerated after PEG-induced fusion between mesophyll protoplasts from the dihaploid potato clone H-8105 and the wild tuberous disease-resistant species S. pinnatisectum, were performed. A single fusion experiment yielded 313 calli, although only two calli produced shoots. From the rooted shoots, two stable clones (PT-01-1 and PT-01-2) exhibiting different vigor and habitat, were developed. The plants of PT-01-1 clone grew slowly in vitro, produced tubers after transfer to soil but did not set flowers. In contrast, the plants of the vigorous clone PT-01-2 produced both tubers and flowers after transfer to soil. The flower and tuber morphology of PT-01-1 and PT-01-2 regenerants was intermediate in comparison to the parental species. Cytological analysis revealed that the PT-01-1 clone was pentaploid and the PT-01-2 clone was tetraploid. The molecular (RAPD) analysis confirmed hybridity of both clones. The preliminary tests on late blight resistance of the hybrids showed no differences with a potato parent.
Starting Page	265
Ending Page	273
Page Count	9
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	27
Issue Number	3
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	potato Solanum pinnatisectum somatic hybridization ploidy RAPD late blight resistance Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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