NDLI: Highly efficient Agrobacterium-based transformation system for callus cells of the C$

Content Provider	Springer Nature Link
Author	Zhao, Shu Zhen Ruan, Yuan Sun, Hong Zhao Wang, Bao Shan
Copyright Year	2008
Abstract	Genetic manipulation technologies have been limited in the halophyte Suaeda salsa L. due to the lack of an efficient transformation system. Here, we examined factors affecting transformation and developed an efficient transformation system at the cell level using S. salsa hypocotyl as starting material. S. salsa hypocotyl explants from 10-day-old seedlings were precultured for 2 days on a hygromycin (hyg)-free callus induction medium (CIM) and then inoculated with Agrobacterium tumefaciens suspension at a concentration of 0.5 at OD$_{600}$ for 5–10 min. After cocultivation with A. tumefaciens for 4 days in the dark, followed by selection on carbenicillin (carb) for 3 days, explants were placed on CIM containing 10 mg l$^{−1}$ hyg and 500 mg l$^{−1}$ carb with three to four consecutive subcultures for up to 45 days. β-Glucuronidase assays showed an average transformation frequency of 62.89%. Gene integration was confirmed by polymerase chain reaction analysis and Northern blot analysis. To our knowledge, this is the first study to show Agrobacterium-mediated transformation in the C$_{3}$ halophyte S. salsa.
Starting Page	729
Ending Page	736
Page Count	8
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	30
Issue Number	5
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-04-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Suaeda salsa A. tumefaciens Hypocotyl GUS Transformation Halophyte Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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