NDLI: Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

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Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

Content Provider	Springer Nature Link
Author	Maciejewska, Urszula Skierski, Janusz S. Szczerbakowa, Anna
Copyright Year	1999
Abstract	The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.
Starting Page	37
Ending Page	43
Page Count	7
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	21
Issue Number	1
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cell suspension DNA content flow cytometry mesophyll protoplast Solanum species Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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