NDLI: Proteome analysis of pollen in the K-type male sterility line 732A and its maintainer 732B in wheat (Triticum aestivum L.) by two-dimensional gel electrophoresis

Please wait, while we are loading the content...

Physiological and antioxidant enzyme gene expression analysis reveals the improved tolerance to drought stress of the somatic hybrid offspring of Brassica napus and Sinapis alba at vegetative stage

Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon

Sunfleck limits the small-scale distribution of endangered Kingdonia uniflora in the natural habitat of subalpine forest proved by its photosynthesis

Isolation and characterization of a plastidic glutamine synthetase (GS) gene in laticifers of Hevea brasiliensis Muell. Arg.

Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

Nitrogen fertilization increases ammonium accumulation during senescence of barley leaves

Rice (Oryza sativa L.) hydraulic conductivity links to leaf venation architecture under well-watered condition rather than PEG-induced water deficit

Adaptation of wheat cells to short-term ozone stress: the impact of α-tocopherol and gallic acid on natural and model membranes

Proteome analysis of pollen in the K-type male sterility line 732A and its maintainer 732B in wheat (Triticum aestivum L.) by two-dimensional gel electrophoresis

The regulation of root growth in response to phosphorus deficiency mediated by phytohormones in a Tibetan wild barley accession

Improving performance of olive trees by the enhancement of key physiological parameters of olive leaves in response to foliar fertilization

Response of Bur and Red oak seedlings to NaCl-induced salinity

Influence of nitrogen and sulfur fertilization on glucosinolate content and composition of horseradish plants harvested at different developmental stages

Developmental regulation of metabolites and low temperature tolerance in lines of crosses between spring and winter wheat

Methyl jasmonate regulated diploid and tetraploid black locust (Robinia pseudoacacia L.) tolerance to salt stress

Morpho-anatomical and physiological adaptations to high altitude in some Aveneae grasses from Neelum Valley, Western Himalayan Kashmir

Foliar application of betaine alleviates cadmium toxicity in maize seedlings

Foliar NK application mitigates drought effects in sunflower (Helianthus annuus L.)

GmSGR1, a stay-green gene in soybean (Glycine max L.), plays an important role in regulating early leaf-yellowing phenotype and plant productivity under nitrogen deprivation

Acta Physiologiae Plantarum : Volume 38, Issue 3, March 2016

Acta Physiologiae Plantarum : Volume 38, Issue 2, February 2016

Acta Physiologiae Plantarum : Volume 38, Issue 1, January 2016

Acta Physiologiae Plantarum : Volume 37

Acta Physiologiae Plantarum : Volume 36

Acta Physiologiae Plantarum : Volume 35

Acta Physiologiae Plantarum : Volume 34

Acta Physiologiae Plantarum : Volume 33

Acta Physiologiae Plantarum : Volume 32

Acta Physiologiae Plantarum : Volume 31

Acta Physiologiae Plantarum : Volume 30

Acta Physiologiae Plantarum : Volume 29

Acta Physiologiae Plantarum : Volume 28

Acta Physiologiae Plantarum : Volume 27

Acta Physiologiae Plantarum : Volume 26

Acta Physiologiae Plantarum : Volume 25

Acta Physiologiae Plantarum : Volume 24

Acta Physiologiae Plantarum : Volume 23

Acta Physiologiae Plantarum : Volume 22

Acta Physiologiae Plantarum : Volume 21

Acta Physiologiae Plantarum : Volume 20

Acta Physiologiae Plantarum : Volume 19

Proteome analysis of pollen in the K-type male sterility line 732A and its maintainer 732B in wheat (Triticum aestivum L.) by two-dimensional gel electrophoresis

Content Provider	Springer Nature Link
Author	Zhang, Fangning Li, Guidong Ding, Qin Wang, Zhen Ma, Xiaofei Zhang, Hongxia Zhang, Zigang Jin, Feng Ma, Lingjian
Copyright Year	2016
Abstract	Male sterility, especially cytoplasmic male sterility (CMS) where no functional pollen is produced, plays an important role in wheat breeding. Some specific proteins are related to male sterility in wheat, especially those present in pollen. To elucidate these sterility-related proteins, we applied two-dimensional gel electrophoresis (2-DE) to the proteomic analysis of pollen from a CMS line (732A) and its maintainer (732B) in wheat (Triticum aestivum L.), where trichloroacetic acid/acetone precipitation was used to extract pollen proteins from the wheat anthers. Analysis using PDQuest detected >350 reproducible protein spots on each 2-DE gel, where pI 4–7. In total, 41 spots were found to be differentially expressed, where 7/41 were identified by MALDI TOF/TOF–mass spectrometry analysis and protein database searching. The proteins comprised NADPH-producing dehydrogenase in the oxidative pentose phosphate pathway, cinnamyl alcohol dehydrogenase, cytosolic 3-phosphoglycerate kinase, caffeic acid O-methyltransferase, ascorbate peroxidase, glutamine synthetase isoform GS1a, and one unknown protein. In the uninucleate stage, all of the identified protein spots were downregulated in 732A, except the unknown protein, whereas there were no obvious differences in 732B. In the binucleate stage, the expression level of glutamine synthetase isoform GS1a did not differ between 732A and 732B, but caffeic acid O-methyltransferase was downregulated in 732A and the other five proteins were upregulated in 732A. These results suggest that male sterility in 732A is related to energy metabolism perturbation, active oxygen accumulation, programmed cell death, the pentose phosphate pathway, and glycolysis.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	38
Issue Number	4
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-03-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anther protein MALDI TOF/TOF–MS Male sterility Two-dimensional gel electrophoresis Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification of changes in Triticum aestivum L. leaf proteome in response to drought stress by 2D-PAGE and MALDI-TOF/TOF mass spectrometry

Proteomic analysis of tomato (Lycopersicon esculentum) pollen

Diversity of Novel Glutenin Subunits in Bread Wheat (Triticum aestivum L.)

Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.

Differential proteomic analysis of polyubiquitin-related proteins in chemical hybridization agent-induced wheat (Triticum aestivum L.) male sterility

Wild Relatives of the Wheat Grain Proteome

Microscopic and Protein Expression Analysis of Grain Weight Variation within the Spike of Triticum aestivum

Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.)

Proteome analysis of cold stress response in Arabidopsis thaliana using DIGE-technology

Proteome analysis of pollen in the K-type male sterility line 732A and its maintainer 732B in wheat (Triticum aestivum L.) by two-dimensional gel electrophoresis

Similar Documents

Identification of changes in Triticum aestivum L. leaf proteome in response to drought stress by 2D-PAGE and MALDI-TOF/TOF mass spectrometry

Proteomic analysis of tomato (Lycopersicon esculentum) pollen

Diversity of Novel Glutenin Subunits in Bread Wheat (Triticum aestivum L.)

Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.

Differential proteomic analysis of polyubiquitin-related proteins in chemical hybridization agent-induced wheat (Triticum aestivum L.) male sterility

Wild Relatives of the Wheat Grain Proteome

Microscopic and Protein Expression Analysis of Grain Weight Variation within the Spike of Triticum aestivum

Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.)

Proteome analysis of cold stress response in Arabidopsis thaliana using DIGE-technology

Proteome analysis of pollen in the K-type male sterility line 732A and its maintainer 732B in wheat (Triticum aestivum L.) by two-dimensional gel electrophoresis