NDLI: De novo transcriptome sequencing of blue honeysuckle fruit (Lonicera caerulea L.) and analysis of major genes involved in anthocyanin biosynthesis

Content Provider	Springer Nature Link
Author	Huo, Jun wei Liu, Peng Wang, Yong Qin, Dong Zhao, Li juan
Copyright Year	2016
Abstract	Blue honeysuckle (Lonicera caerulea L.) is an economically important berry because its flavonoid and anthocyanin contents are generally higher than those of other fruits. However, research on anthocyanin accumulation in blue honeysuckle is limited by insufficient genome information. In this paper, Illumina HiSeq™ 2000 platform was used for the transcriptome sequencing of blue honeysuckle fruit in two periods (green-ripe and veraison periods). This platform was also used to obtain more than 116 million sequencing reads. Those reads were de novo assembled and produced 66,610 All-unigenes. After a comparison between currently available public databases (NR, Swiss-prot, KEGG, COG, and GO) and obtained annotated unigenes, 43,202 unigenes (64.86 %) showed similarity to known protein sequences (E value ≤0.00001). The KEGG pathway indicated that some genes related to anthocyanin biosynthesis existed mainly in the secondary metabolic pathway of the samples. A series of enzymes related to anthocyanin biosynthesis was identified in NR annotation; some candidate genes and their metabolic pathways were also found. We conducted a preliminary analysis on the expression of some important enzymes (PAL, CHS, F3H, ANS, and DFR) and transcription factors (MYB, bHLH, and WD40) related to anthocyanin biosynthesis through quantitative real-time PCR to explore the biosynthesis process of anthocyanin and the accuracy of sequencing results. Information on blue honeysuckle transcriptome can serve as an important reference for breeding and production.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	38
Issue Number	7
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-06-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Blue honeysuckle Anthocyanin Transcriptome Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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