NDLI: An improved method of DNA purification from secondary metabolites rich medicinal plants using certain chaotropic agents

Content Provider	Springer Nature Link
Author	Ghadia, Bansi Singh, Anil Kumar Khatnani, Tannu Hirpara, Mansi Patel, Shivani Joshi, Preetam
Copyright Year	2016
Abstract	The presented work describes good quality DNA isolation method from mature leaves of some medicinally important plant species, viz. Asparagus racemosus, Withania somnifera, Abrus precatorius, Commiphora wightii and Carissa carandas. These plants hold immense medicinal values due to presence of certain secondary metabolites like polyphenols, terpenes, flavonoids, alkaloids, gums, resins, etc. Although these metabolites are accountable for important medicinal properties and authorize these plants to precedence over others, the same compounds disappoint the researcher while isolating high quality DNA. To overcome this problem, we propose a simple method in which DNA is adroitly bounded to diatomaceous earth in a solution of different chaotropic agent and alienated from intrusive compounds. Presented method affirms that secondary products, along with polysaccharides and proteins, can be perceptibly reduced by using silica matrix along with chaotropic agents. The described method is fast, simple and highly reliable for the isolation of DNA from obstinate plant species.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	38
Issue Number	8
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-07-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA isolation Medicinal plants CTAB Chaotropic agents Diatomaceous earth Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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