NDLI: TaPT2, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), is crucial in plant Pi uptake under phosphorus deprivation

Content Provider	Springer Nature Link
Author	Guo, Chengjin Guo, Li Li, Xiaojuan Gu, Juntao Zhao, Miao Duan, Weiwei Ma, Chunying Lu, Wenjing Xiao, Kai
Copyright Year	2014
Abstract	Acquisition of inorganic phosphate (Pi) by plant roots is performed by phosphate transporters (PTs) located at the cytoplasmic membranes of epidermal cells and root hairs. A Triticum aestivum PT gene denoted as TaPT2 was functionally characterized in this study. TaPT2 is highly similar to TtPT2 and HvPT2, two PHT1 family genes in T. aestivum/Thinopyrum intermedium and barley, respectively. TaPT2 is 1,802 bp long at the cDNA level; it encodes a 525-amino acid polypeptide with a molecular weight of 57.5 kDa and an isoelectric point of 8.65. TaPT2 contains 12 conserved membrane-spanning domains and is transported to the cytosolic membrane after endoplasmic reticulum sorting. Functional complement analysis revealed that TaPT2 endowed Pi transporter activities in a yeast mutant that is defective in Pi uptake, with high-affinity Pi acquisition. TaPT2 transcripts were specifically detected in the roots. The transcripts were upregulated under Pi deprivation and downregulated under Pi sufficiency. These results suggest that TaPT2 expression is associated with external Pi concentration. Transgene analysis revealed that TaPT2 overexpression or knockdown did not regulate plant dry mass production, Pi acquisition, and photosynthetic capacity under Pi sufficiency. Under Pi deprivation, TaPT2 overexpression increased plant dry mass accumulation, total P content per plant, and photosynthetic efficiencies, whereas TaPT2 downregulation reduced plant dry mass, accumulative P amount, and photosynthetic parameters. These results collectively suggest that TaPT2 is a high-affinity PHT1 member that has important functions in mediating plant Pi uptake under Pi deprivation. TaPT2 can serve as a useful gene resource for the improvement of phosphorus use efficiency in cereals under Pi deprivation.
Starting Page	1373
Ending Page	1384
Page Count	12
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	36
Issue Number	6
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-04-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Wheat (Triticum aestivum L.) Phosphate transporter Gene expression Transgene analysis Plant growth Phosphate acquisition Phosphorus deprivation Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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