NDLI: Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses

Content Provider	Springer Nature Link
Author	Wang, Lijuan Ma, Fei Xu, Sheng Zheng, Tianqing Wang, Ren Chen, Huiping Shen, Wenbiao
Copyright Year	2013
Abstract	Heme oxygenase (HO; EC 1.14.99.3) is an important enzyme that yields biliverdin IXα (BV), carbon monoxide and iron. At least two kinds of HO subfamilies exist in plants. Our previous report revealed that rice (Oryza sativa L.) HO1 (SE5, also named as OsHO1), a major subfamily of HO, is an oxidative stress–response protein, especially upon paraquat exposure. However, whether there exists rice HO2, another subfamily of HO, is still unknown. In the present study, a rice HO2 gene (named as OsHO2) was cloned and characterized. Its genomic sequence consists of four exons and three introns, and encodes a 36.5 kDa protein precursor with a 4.9 kDa N-terminal transit leader peptide. Further results showed that OsHO2 has a conserved HO signature sequence and shares a high amino acid sequence similarity with other identified plant HO2s. The recombinant mature OsHO2 (mOsHO2) protein expressed in Escherichia coli did not exhibit HO activity, which was in contrast with that of mOsHO1. The results of subcellular localization of OsHO2 demonstrated that it was most likely localized in the chloroplasts. Real-time RT-PCR experiment revealed that although OsHO2 mRNA is a much less abundant than that of OsHO1, both of them were expressed in all tested tissues. Importantly, OsHO2 transcripts could be differentially induced by hemin (a substrate of HO), paraquat (in particular), and NaCl treatments. Together, the results suggested that OsHO2 might act downstream in the signal transduction pathways following abiotic stresses in rice.
Starting Page	893
Ending Page	902
Page Count	10
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	36
Issue Number	4
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-12-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress responses Gene expression Heme oxygenase 2 (HO2) Prokaryotic expression Rice (Oryza sativa L.) Subcellular localization Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

NaCl-induced heme oxygenase in roots of rice seedlings is mediated through hydrogen peroxide

Heme oxygenase 1 and abiotic stresses in plants

Young Leaf Chlorosis 2 encodes the stroma-localized heme oxygenase 2 which is required for normal tetrapyrrole biosynthesis in rice

Cloning and characterization of NtSnRK2.7 and NtSnRK2.8 genes involved in abiotic stress responses from Nicotiana tabacum

Cloning and functional analysis of pale-green leaf (PGL10) in rice (Oryza sativa L.)

Expression Profiles of Stress Responsive Genes in Rice (Oryza Sativa L.) Under Abiotic Stresses

Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)

Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses

Effects of two species of inorganic arsenic on the nutrient physiology of rice seedlings

Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses

Similar Documents

NaCl-induced heme oxygenase in roots of rice seedlings is mediated through hydrogen peroxide

Heme oxygenase 1 and abiotic stresses in plants

Young Leaf Chlorosis 2 encodes the stroma-localized heme oxygenase 2 which is required for normal tetrapyrrole biosynthesis in rice

Cloning and characterization of NtSnRK2.7 and NtSnRK2.8 genes involved in abiotic stress responses from Nicotiana tabacum

Cloning and functional analysis of pale-green leaf (PGL10) in rice (Oryza sativa L.)

Expression Profiles of Stress Responsive Genes in Rice (Oryza Sativa L.) Under Abiotic Stresses

Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)

Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses

Effects of two species of inorganic arsenic on the nutrient physiology of rice seedlings

Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses