NDLI: High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

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High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

Content Provider	Springer Nature Link
Author	Memon, Saba Ambreen Hou, Xilin Zhu, Bo Wolukau, Joseph N.
Copyright Year	2009
Abstract	The objective of the present work was selection of cultivar and suitable medium for regenerating shoots from leaf segments of non-heading Chinese cabbage. We evaluated six types of supplemented media with 2.0, 5.0 and 10.0 mg l$^{−1}$ 6-BA; 1.0 and 2.0 mg l$^{−1}$ TDZ; 0.1, 0.3, 0.5, 0.8 and 1.0 mg l$^{−1}$NAA; 3.0, 5.0 and 7.5 mg l$^{−1}$AgNO$_{3}$; 0.01 mg l$^{−1}$ 2–4, D and 4.0 mg l$^{−1}$ KT for shoot regeneration and six cultivars “Sanchidaye”, “Liuchuandasuomian”, “Qingyou 4”, “Liangbaiye”, “AiKang 5” and “Hanxiao F3”, furthermore for root formation three types of supplemented media with 0.2, 0.3, 0.5 mg l$^{−1}$ NAA, and for survival rate two types of base media: turf + vermiculite + manure (1:2:0.2) and soil + vermiculite (1:2). Culturing leaf segments on MS medium supplemented with 2 mg l$^{−1}$ TDZ; 0.5 mg l$^{−1}$ NAA and 7.5 mg l$^{−1}$ AgNO$_{3}$ gave the highest number of shoots per leaf segment (66) while roots were best formed on the medium supplemented with 0.2 mg l$^{−1}$ NAA. Survival rate was highest (61.6%) in the turf: vermiculite: manure (1:2:0.2) medium. The highest percentage of responding leaf segments, number of shoots per leaf segment, rooting percentage and survival rate were observed in “Liuchuandasuomian”. The plantlets were transferred to the soil and grown into mature plants in pots. These results could be used for preliminary selections of cultivars to transfer disease resistance (Bt) gene through agrobacterium in non-heading Chinese cabbage.
Starting Page	1191
Ending Page	1196
Page Count	6
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	31
Issue Number	6
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-09-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Non-heading Chinese cabbage Micropropagation Leaf explant Shoot generation Cultivar In vitro Plant growth regulators Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

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