NDLI: Effects of dynamic changes of nutrients on adventitious roots growth and periplocin accumulation in culture of Periploca sepium Bunge

Content Provider	Springer Nature Link
Author	Yin, Shuangshuang Gao, Wenyuan Wang, Juan Zhang, Jian Man, Shuli Jing, Song Song Liu, Hui
Copyright Year	2013
Abstract	In this study, the dynamics of biomass production, accumulation of periplocin, medium conditions and consumption of carbon, nitrogen and phosphate were investigated in adventitious roots culture of Periploca sepium in shake flasks over a period of 4 weeks. The biomass reached the maximum peak on day 24 (2.46 and 0.213 g of fresh and dry weight, respectively). Similarly, periplocin production got to a peak of 0.083 mg g$^{−1}$ on day 24, simultaneously. PH in medium had a decrease tendency at the beginning and then remained stable around 4.0, however, EC declined during the whole culture with the nutrients consumed. Sucrose was almost used up on the first 12 days which led to the increase in glucose and fructose. In case of nitrogen, consumption of ammonium is faster than nitrate at the beginning. Phosphate was almost consumed during the first 8 days. Based on the nutrients consumption, the adding time of nutrients (1/2 MS medium and 30 g L$^{−1}$ sucrose) was investigated and it obtained highest content of periplocin (0.106 mg g$^{−1}$) and yield (0.513 mg L$^{−1}$) on day 12.
Starting Page	3085
Ending Page	3090
Page Count	6
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	35
Issue Number	11
e-ISSN	18611664
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-07-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Periploca sepium Adventitious root Periplocin Medium condition Nutrients consumption Fed-batch Plant Physiology Plant Genetics & Genomics Plant Biochemistry Plant Pathology Plant Anatomy/Development Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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