NDLI: Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress

Content Provider	Springer Nature Link
Author	Daldoul, Samia Toumi, Imen Reustle, Götz M. Krczal, Gabi Ghorbel, Abdelwahed Mliki, Ahmed Höfer, Michael U.
Copyright Year	2011
Abstract	The expression of a gene originally identified by means of a salt stress-associated partial cDNA showing a genotype dependent response was studied upon exposure to salt and drought stress and during seed development of grapevines. The complete open reading frame has been cloned and the corresponding untranslated regions have been characterized by 5′- and 3′-RACE leading to a refinement and correction of the predicted gene model in the recent 12× Vitis genome assembly. The open reading frame of the single copy gene designated Vv-α-gal/SIP encodes for a polypeptide of 774 amino acids with a calculated molecular mass of 84.9 kDa. Protein sequence comparisons suggest that the gene belongs to a growing family of plant-specific alkaline α-galactosidases. 5′-RACE identified a not yet documented transcript species derived from the same gene locus which does not lead to the formation of a full-length transcript. The “aberrant” transcript harbours an alternative 5′-UTR and originates from an internal transcriptional start site 1.4 kb downstream of the predominant transcriptional start site. Levels of transcripts containing the different 5′-UTRs were compared under salt stress conditions.
Starting Page	731
Ending Page	742
Page Count	12
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	34
Issue Number	2
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-11-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alkaline alpha-galactosidase Alternative transcript Differential gene expression Drought stress Grapevine Salt stress Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Anatomy/Development Agriculture Plant Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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