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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shi, Shouguo Ma, Fengwang Li, Yonghong Feng, Fengjuan Shang, Zengzhen |
| Copyright Year | 2011 |
| Abstract | In China, the native Lanzhou lily (Lilium davidii var. unicolor) is used as an edible lily and is an important source of ascorbic acid. However, an efficient transformation system has not been developed for this important crop. Here, we describe the first successful genetic transformation mediated by particle bombardment in this lily variety. We used pCAMBIA-GLDH, which contains the l -galactono-1, 4-lactone dehydrogenase gene from apple, a hygromycin phosphotransferase gene as a selectable marker, and an intron-containing β-glucuronidase gene as a reporter. In all, 35 transgenic lines were selected via GUS assays and PCR; from these, eight were confirmed by Southern analyses. Transgenic plants were transferred to soil and grown under greenhouse conditions. The levels of reduced ascorbate and total ascorbate (reduced ascorbate + oxidized ascorbate) in transgenic plants were found to be two to seven times those in non-transgenic controls. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | |
| ISSN | 10545476 |
| Journal | In Vitro Cellular & Developmental Biology - Plant |
| Volume Number | 48 |
| Issue Number | 1 |
| e-ISSN | 14752689 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-07-26 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Ascorbate Lanzhou lily Particle bombardment Transformation Plant Breeding/Biotechnology Developmental Biology Plant Genetics & Genomics Cell Biology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Plant Science Biotechnology |
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