NDLI: Relationships between nutrients and plant density in liquid media during micropropagation and acclimatization of turmeric

Content Provider	Springer Nature Link
Author	Adelberg, Jeffrey Driesse, Todd Halloran, Sean Bridges, William C.
Copyright Year	2013
Abstract	To improve micropropagation and acclimatization of turmeric (Curcuma longa L.), we evaluated the effects of media volume, plant density, macronutrient ion concentrations, cationic ratios [NH$_{4}$ $^{+}$]/[K$^{+}$], and sucrose concentration. Multiplication was highest with low bud density. Yield of new plants was highest with high bud density, the most sucrose, the highest concentration of macronutrients, and the greatest volume of medium. However, maximum plant size required low-density, reduced sucrose and elimination of NH$_{4}$ $^{+}$. The largest plants grew quickest during greenhouse acclimatization when macronutrients were lowered to 20 mM. In a follow-up experiment, media volume was set at 40 mL with 5% sucrose with NH$_{4}$ $^{+}$ reduced to 5 mM, and the effects of varying P, Ca, Mg, KNO$_{3}$, and bud densities were assessed. The largest plants were produced at low density. More importantly, at high density the optimal concentrations of P, Ca, Mg, and KNO$_{3}$ predicted plant size that was nearly equal to the maximum value from low-density cultures in the prior experiment. Growth of plants during greenhouse acclimatization was increased by modifications of in vitro medium with plants cultured with 3.32 mM P, 4.5 mM Mg, and 37 mM KNO$_{3}$ predicted to grow most rapidly. The effect of starter fertilizer in the greenhouse mix was much less than the effects of P, Mg, and KNO$_{3}$ in vitro. These results showed (1) optimal media formulae for different stages of micropropagation and (2) process-related factors such as plant density and media volume affected the optimal nutrient concentrations.
Starting Page	724
Ending Page	736
Page Count	13
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	49
Issue Number	6
e-ISSN	14752689
Language	English
Publisher	Springer US
Publisher Date	2013-11-14
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Curcuma longa d-Optimal Mineral nutrition Multifactor Response surface model Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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