NDLI: A simple wheat haploid and doubled haploid production system using anther culture

Please wait, while we are loading the content...

2004 SIVB Congress Symposium Proceeding: Mechanisms of somatic embryogenesis in carrot suspension cultures—Morphology, physiology, biochemistry, and molecular biology

Using a competent tissue for efficient transformation of sugarbeet (Beta vulgaris L.)

Transfer and expression of nptII and bar genes in cucumber (Cucumis satavus L.)

A simple wheat haploid and doubled haploid production system using anther culture

Somatic embryogenesis, encapsulation, and plant regeneration of Rotula aquatica lour., a rare rhoeophytic woody medicinal plant

Regeneration from long-term embryogenic callus of the Rosa hybrida cultivar kardinal

Modes of regeneration in Pelargonium × hortorum (Geraniaceae) and three closely related species

The effect of phytohormones on growth and artemisinin production in Artemisia annua hairy roots

Direct and indirect somatic embryogenesis on cotyledon explants of Quassia amara L., an antileukaemic drug plant

Effects of the growth retardant paclobutrazol on large-scale micropropagation of daylily (Hemerocallis spp.)

Cryopreservation of chestnut by vitrification of in vitro-grown shoot tips

Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets

In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight & Arn., a threatened plant endemic to Southern India

Micropropagation of socotran fig, Dorstenia gigas schweinf. Ex Balf. F. —A threatened species, endemic to the island of Socotra, Yemen

New contributions to propagation of pineapple (Ananas comosus L. Merr) in temporary immersion bioreactors

In Vitro Cellular & Developmental Biology - Plant : Volume 40

In Vitro Cellular & Developmental Biology - Plant : Volume 39

In Vitro Cellular & Developmental Biology - Plant : Volume 38

In Vitro Cellular & Developmental Biology - Plant : Volume 37

In Vitro Cellular & Developmental Biology - Plant : Volume 36

In Vitro Cellular & Developmental Biology - Plant : Volume 35

In Vitro Cellular & Developmental Biology - Plant : Volume 34

In Vitro Cellular & Developmental Biology - Plant : Volume 33

A simple wheat haploid and doubled haploid production system using anther culture

Content Provider	Springer Nature Link
Author	Kim, Kyung Moon Baenziger, P. Stephen
Copyright Year	2005
Abstract	Wheat (Triticum aestivum L.) haploids and doubled haploids have been used in breeding programs and genetic studies. Wheat haploids and doubled haploids via anther culture are usually produced by a multiple step culture procedure. We improved a wheat haploid and doubled haploid production system via anther culture in which plants are produced from microspore-derived embryos using one medium and one culture environment. In the improved protocol, tillers of donor plants were pretreated at 4°C for 1–2 wk before anthers were plated on a modified 85D12 basal medium with phenylacetic acid (PAA) and zeatin and cultured at 30°C with a 12-h daylength (43 μEs$^{−1}$m$^{−2}$) in an incubator. Microspore-derived embryos developed in 2–3 wk and the plants were produced 3–4 wk after anther plating. In the improved system, as much as 53% of the anthers of Pavon 76 were responsive with multiple embryos. For plant regeneration, as many as 22 green and 25 albino plants were produced from 100 anthers. Sixty-five green plants were grown to maturity and 32 (49%) plants were fertile and produced seeds (indicating spontaneous chromosome doubling) while 33 plants did not produce seed. Of five Nebraska breeding lines tested using the protocol, NE96675 was very responsive and the other lines less so, indicating that the protocol is genotype-dependent.
Starting Page	22
Ending Page	27
Page Count	6
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	41
Issue Number	1
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	microspore one step culture plant tissue culture Triticum aestivum Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry

Microspore culture in wheat (Triticum aestivum) – doubled haploid production via induced embryogenesis

Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.)

Production of doubled haploid wheat lines (Triticum aestivum L.) using anther culture technique

Effects of colchicine on anther and microspore culture of bread wheat (Triticum aestivum L.)

The discovery of haploid production by anther culture

Anther culture for haploid and doubled haploid production

A simple wheat haploid and doubled haploid production system using anther culture

Similar Documents

Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry

Microspore culture in wheat (Triticum aestivum) – doubled haploid production via induced embryogenesis

Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.)

Production of doubled haploid wheat lines (Triticum aestivum L.) using anther culture technique

Effects of colchicine on anther and microspore culture of bread wheat (Triticum aestivum L.)

The discovery of haploid production by anther culture

Anther culture for haploid and doubled haploid production

A simple wheat haploid and doubled haploid production system using anther culture