NDLI: Effect of silver nitrate on multiple shoot formation of Virginia-type peanut through shoot tip culture

Content Provider	Springer Nature Link
Author	Ozudogru, Elif Aylin Ozden Tokatli, Yelda Akcin, Abdulkadir
Copyright Year	2005
Abstract	A protocol for micropropagation of Virginia-type peanut plants, an ancient crop of the New World, is reported. This study was conducted to explore the effect of silver nitrate (AgNO$_{3}$), alone or in combination with growth regulators, on multiple shoot formation from shoot tip culture. Incorporation of AgNO$_{3}$ into the medium, without growth regulators, induced regeneration of the explants (which did not develop at all in the AgNO$_{3}$-free medium), and stimulated the emergence of axillary shoots. When AgNO$_{3}$ was added in combination with cytokinins and α-naphthaleneacetic acid (NAA), maximum average shoot number per regenerating explant was recorded (6.3) in Murashige and Skoog (MS) medium containing 33 μM 6-benzyladenine, 5.3 μM NAA, and 23.54 μM AgNO$_{3}$. Moreover, AgNO$_{3}$ showed a positive and marked effect on both shoot elongation and the reduction of callus proliferation from the basal ends of shoot tips. Following a period of elongation, the shoots were rooted in hormone-free Ms medium, showing no residual effects due to the long-term culture in AgNO$_{3}$-containing media. Acclimatization was easily obtained after plantlets were transferred to pots under greenhouse conditions, with 90% survival.
Starting Page	151
Ending Page	156
Page Count	6
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	41
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arachis hypogaea L. micropropagation multiple shoot formation peanut shoot tip silver nitrate Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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