NDLI: Developing an improved In vitro propagation system for slow-growing species using garcinia mangostana L. (mangosteen)

Content Provider	Springer Nature Link
Author	Huang, Li Chun Huang, Bau Liang Wang, Chiu Hui Kuo, Ching I Murashige, Toshio
Copyright Year	2000
Abstract	This investigation disclosed that evaluation of tissue culture parameters of slowly developing species (e.g. Garcinia mangostana) requires monitoring of treatments through two or more successive, relatively long passages. Two 8-wk passages were necessary to observe differences in phytohormone effects. Photoperiod and temperature effects were not clearly evident until tissues had been cultured through three passages; the optimal photoperiod and temperature for shoot proliferation could not be established until after the fifth passage. Our investigation revealed that no auxin supplementation was necessary for bud primordium differentiation in cotyledon explants or proliferation of regenerated shoots. The optimum N$^{6}$-benzyladenine concentration for primordium differentiation was 13.3 μM, and for shoot proliferation ranged from 4.4 to 13.3 μM. Continuous culturing in an 8-h photoperiod at 30°C resulted in progressively intensified degeneration of shoots after three passages. In contrast, successive passages in a 16-h photoperiod/26°C regimen enabled sustained regeneration of shoots. The shoots rooted at a rate of 85% when precultured for 3 d in a medium containing 4921.3 μM indole-3-butyric acid, or 10 d at 492.1 μM, then cultured for two 8-wk passages in phytohormone-free medium. Following acclimatization by gradually lowering the relative humidity in the growth chamber, rooted shoots survived transfer to the greenhouse at a rate of 95%.
Starting Page	501
Ending Page	504
Page Count	4
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	36
Issue Number	6
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	mangosteen micropropagation plant regeneration two-step rooting acclimatization Plant Biochemistry Cell Biology Developmental Biology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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