NDLI: The establishment of axenic seed and embryo cultures of endangered Hawaiian plant species: special review of disinfestation protocols

Content Provider	Springer Nature Link
Author	Sugii, Nellie C.
Copyright Year	2010
Abstract	More than one third of the USA’s federally listed endangered and threatened plants are endemic to Hawaii. Because of the accelerating rate of environmental pressures resulting from habitat degradation to loss, the Hawaiian Rare Plant Program (HRPP, Lyon Arboretum, Honolulu, Hawaii) utilizes in vitro culture and seed storage to rescue, recover, and restore Hawaii’s most critically endangered native plant taxa. These in vitro methods complement existing ex situ and in situ efforts in the state. Wild-collected seeds tend to be hard to clean, and obtaining contamination-free seeds or embryos without damage or over sterilization is one of the most difficult obstacles in the successful establishment of in vitro cultures. Loss of rare and valuable propagation material occurs when seed samples succumb to contamination or mortality during the initial disinfestation stage. This study reviews pre-treatments and provides examples of three general disinfestation protocols successfully used in the HRPP to establish axenic ovule and embryo cultures of some endangered Hawaiian species. Three disinfestation protocols are described: bleach sterilization, gas sterilization, and ethanol dip and flame. Since 1998, the HRPP has been successful in establishing in vitro cultures of 135 endangered Hawaiian plant taxa representing 30 genera.
Starting Page	157
Ending Page	169
Page Count	13
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	47
Issue Number	1
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Axenic Disinfestation Embryo Endangered Hawaiian In vitro Seed Plant Genetics & Genomics Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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