NDLI: Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper

Content Provider	Springer Nature Link
Author	Tang, Kexuan Hu, Quanan Sun, Xianfen Wan, Bingliang Qi, Huaxiong Lu, Xinggui
Copyright Year	2001
Abstract	Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromycin phosphotransferase gene (hpt), the reporter β-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. Thirty-five independent transgenic rice plants were regenerated from 177 bombarded calluses. Eighty-three percent of the transgenic plants contained all three genes, as revealed by Southern blot analysis. Western blot analysis revealed that 23 out of 29 gna-containing transgenic plants expressed Galanthus nivalis agglutinin (GNA) (79%) at various levels, with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of all three transgenes (gna, hpt and gusA) in the R2 progeny. Amongst the R2 generation two independent homozygous lines were identified that expressed all three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing the survival, overall fecundity of BPH, retarding development, and decreasing the feeding of BPH. These BPH-resistant lines have been incorporated into a rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH.
Starting Page	334
Ending Page	340
Page Count	7
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	37
Issue Number	3
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cotransformation Oryza sativa L particle bombardment segregation analysis snowdrop lectin gene (gna) transgenic rice Plant Biochemistry Cell Biology Developmental Biology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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Co-transformation of gene expression cassettes via particle bombardment to generate safe transgenic plant without any unwanted DNA

Fine mapping of brown planthopper (Nilaparvata lugens Stål) resistance gene Bph28(t) in rice (Oryza sativa L.)

Transgenic rice plants expressing the snowdrop lectin gene (gna) exhibit high-level resistance to the whitebacked planthopper (Sogatella furcifera)

Identification and mapping of gene(s) for resistance to brown planthopper Nilaparvata lugens (Stal.) in rice (Oryza sativa L.)

Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice

Mapping of brown planthopper [Nilaparvata lugens (Stål)] resistance gene (bph5) in rice (Oryza sativa L.)

GENETIC ANALYSIS OF RESISTANCE TO BROWN PLANTHOPPER (Nilapartava lugens Stal ) IN RICE (Oryza sativa L.)

High-resolution mapping of brown planthopper (BPH) resistance gene Bph27(t) in rice (Oryza sativa L.)

GENETIC ANALYSIS OF RESISTANCE TO BROWN PLANTHOPPER Nilaparvata lugens STAL IN RICE (Oryza sativa L.)

Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper

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